ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (2): 278-283.doi:

• 研究简报 • Previous Articles     Next Articles

Differentiation of Porcine TLR4 Gene mRNA Expression between Resistantand Sensitive Resource Populations to ETEC F18

1.Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province,College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China；2. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China；3. Jiangsu Engineering Research Centre for Molecular Breeding of Breeder Pig, Changzhou 213149, China；4. Jiangsu（Yangzhou）Public Technical Service Centre for Efficient and Healthy Culture Technology in Largescale Swine Farms, Yangzhou 225009, China   

  1. 1.Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province,College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China；2. College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China；3. Jiangsu Engineering Research Centre for Molecular Breeding of Breeder Pig, Changzhou 213149, China；4. Jiangsu（Yangzhou）Public Technical Service Centre for Efficient and Healthy Culture Technology in Largescale Swine Farms, Yangzhou 225009, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-25 Published:2011-02-25

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Abstract: This study was conducted to detect the expression of TLR4 gene in various tissues of pig, and then to analyze the expression difference between resistant and sensitive resource populations to ETEC F18, which aimed to discuss the role that TLR4 gene played in immunity and resistance to ETEC F18. Porcine TLR4 mRNA was amplified by SYBR-GreenⅠ semi-quantitative RT-PCR, total RNA was extracted from 11 organizations of sixteen 35-day-old piglets(eight ETEC F18 resistant piglets and eight F18 sensitive piglets) including heart, liver, spleen,and so on. Using GAPDH as an inner control gene, the relative TLR4 gene mRNA expression level in various tissues and then in resistant and sensitive resource populations to ETEC F18 was calculated according to the fluorescence threshold (Ct value). The results showed that TLR4 gene was expressed in all tissues and higher expression levels were detected in immune organs like lung, lymph node, kidney, spleen and thymus gland. The mRNA expression of TLR4 gene in sensitive individuals were higher than that in resistant individuals generally, the ratio of sensitive individuals and resistant individuals in all tissues apart from stomach ranged from 1.083 to 2.980. In tissues of lung, lymph node, kidney and spleen, the mRNA expression of TLR4 gene in sensitive individuals were significant higher than that in resistant individuals (P<0.05). The results speculated that TLR4 gene function may be related to innate immune recognition, particularly have a certain impact on porcine Gram-negative bacteria such as ETEC F18 etc The resistance to ETEC F18 in pig is related to down regulation of mRNA expression of TLR4 gene to some degree.