Abstract: Study on content of SOD in whole blood of sika deer from different physiological period. The blood of male sika deer in different physiological periods was centrifugated, and then using pyrogallol method to detect the content of SOD in blood corpuscle, plasma, dissolve blood and cell membrane, lastly estimated content of SOD in whole blood. The results showed that the highest content of SOD ((1 007.07±11.03) U·mL^-1) appears during the time of developing antler, besides we found that there was a remarkable discrepancy between developing antler period and remaining two periods(P<0.01). But no significant difference there was between preantler period and service period(P>0.05). The research demonstrated that there were some certainly correlative bewteen the change of SOD content and being different physiological periods. The developing anlter period have the highest SOD content in deer blood, that could be to improve and increase the medicine value, especially the function of anti-aging. In addition, during various physiological stages, we found the dissolve blood should be the best raw material for producing deer blood against aging, because of the highest SOD content has had been happening in it.