Abstract: To explore the evolutionary patterns of avian influenza virus (AIV) in poultry in Eastern China, epidemiological surveillance of AIVs was carried out in Eastern China, and one H11N2 subtype avian influenza virus, A/duck/Jiangxi/k0701/2009, was isolated from domestic duck in a live bird market. The analysis of complete genome sequences and genetic evolution showed that all eight segments of A/duck/Jiangxi/k0701/2009(H11N2) belong to Eurasian lineage, but genetic recombination was observed between A/duck/Jiangxi/k0701/2009(H11N2) and AIVs in some regions in Asia and Europe in varying degrees and extensively. Moreover, the PB2 and NS gene may be originated from A/mallard/Korea/GH170/2007(H7N7), NA gene may have the same origin with NA of HPAIV A/duck/Hebei/0710/2009(H5N2), and NP, PA, PB1 genes may have the same origin with A/Muscovy duck/Thailand/CU-LM1984/2009(H4N9). Our results indicated that A/duck/Jiangxi/k0701/2009 may be a multi-origin reassortant virus, and may play an important role in the evolution of HPAIVs.