ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (2): 224-227.doi:

• 预防兽医 • Previous Articles     Next Articles

Evaluation on ALV Infection in Fertilized Eggs from A Wan-nan Yellow-feather Parent Broiler Breeder Flock

WANG Bo1, LI Qing-yuan1, LIU Shao-qiong1, ZHANG Yong-guang3, CUI Zhi-zhong1,2* , SUN Shu-hong1,2*   

  1. 1.College of Veterinary Medicine， Shandong Agricultural University, Taian 271018, China;2.Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian 271018, China;3. Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-25 Published:2011-02-25

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Abstract: In the present study, the infection of avian leukosis virus (ALV) in parent breeder flock was evaluated by the inspection of fertilized eggs. 120 eggs were obtained for a flock of Wan-nan yellow-feather parent layers. 40 eggs were hatching for 9 to 11 days for the preparation of CEF, which were cultured for 10 days and the supernatants were collected for the measurement of p27 antigen by ELISA. The egg white was obtained from the other 80 eggs and used for the direct measurement of p27 antigen or indirect measurement after inoculation with CEF (DF1) and cultured for 10 days.The results showed that ALV positive rate (10/36) was higher in the measurement with cultured CEF than that in the determination with egg white (17/80) or egg white inoculated with CEF (7/80). The ALV-J positive rate in DF1 positive samples was 6/7 by the measurement of IFA with JE9, a monoclonal antibody to ALV-J. The positive rates of ALV-J and ALV-AB in egg yolk antibody were 18/80 and 1/80, respectively. The results showed that the parent flock was infected with ALV-J. The result suggests that the measurement of ALV antigen from eggs is an available method for the detection of ALV infection.