ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (8): 944-950.doi:

• 遗传繁育 • Previous Articles     Next Articles

Association of 14 Polymorphic Sites of the DRD2 Gene with Chicken Egg Laying Traits

XU Hai-ping1, ZHOU Min2, FANG Mei-xia1, 3, ZENG Hua1, NIE Qing-hua1,
ZHANG De-xiang1, ZHANG Xi-quan1*   

  1. 1. College of Animal Science, South China Agricultural University, Guangzhou 510642, China;2. Department of Biological Technology, Jiangxi Institute of Education, Nanchang 330029, China;3. Department of Laboratory Animal Science, Medical College of Jinan University, Guangzhou 510632, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-08-20 Published:2010-08-20

PDF (PC)

779

Abstract: The purpose of this study was to analyze the associations of the DRD2 gene with chicken egg laying traits and find important markers for these traits. Fourteen polymorphic sites of this gene were selected, and the association analysis was performed in 644 chickens of Ningdu Sanhuang female line by PCR-RFLP and sequencing methods. The results showed that 4 mutation sites in the 5′regulatory region of the DRD2 gene, G-38560C, T-38326G, T-32751C and A-16105G, were significantly associated with the age of first egg (AFE) (P<0.05 or P<0.01). A-16105G was also significantly associated with total egg number at 300 days (EN300) (P<0.05). The haplotype block consisting of G-38560C, G-38544A, I-38463D and T-38326G was significantly associated with AFE (P<0.05). It was suggested that G-38560C, T-38326G, T-32751C and A-16105G on the DRD2 gene and the haplotype block consisting of G38560C, G-38544A, I-38463D and T-38326G could be used as effective molecular markers in marker assisted selection (MAS) for egg laying traits.