Abstract: This experiment was conducted to study the effect of overfeeding on Perilipin mRNA level in goose. In this study, the Landes goose and Sichuan White goose were used to clone the partial gene sequence of Perilipin, and the effect of overfeeding on the transcriptional level of Perilipin in liver was researched. The result indicated that the obtained Perilipin gene sequence was 277 bp, and had high similarity with other species. The mRNA expression of Perilipin gene was detected in different tissues of Sichuan White goose, and its expression level was higher in abdominal adipose and subcutaneous adipose tissues. Overfeeding markedly increased the mRNA expression of Perilipin gene in the liver of two breeds. In control group, the mRNA level of Perilipin in liver of Landes and Sichuan White goose was not different; compared with control group, in overfeeding group, the mRNA level of Perilipin in liver was markedly higher in Sichuan White goose and Landes goose, and there was significant difference at the mRNA level of Perilipin in liver between the two breeds. The mRNA abundance of Perilipin was positively correlated to the relative weight of liver, TG levels and total fatty acids in liver after overfeeding, and the correlation in Landes goose was stronger than that in Sichuan White goose. It was concluded that overfeeding induced the significant increase of Perilipin mRNA level in goose liver, and the effect of overfeeding was different between the two breeds.