Abstract: From October 2008 to September 2009, 1 075 cloaca swab samples were collected from ducks in the Live-birds market, and 24 strains of H3 AIVs were isolated. The analysis of complete gene sequences and genetic evolution showed that two H3N2 avian influenza virus were both low pathogenic avian influenza virus and shared high genetic homology with the strains isolated from swine, whereas low to the strains from human. There are various degrees genetic recombination of the genes except HA, among different subtypes in different regions and different hosts, respectively.