ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (10): 1359-1363.doi:

• 研究简报 • Previous Articles    

Cloning and Sequence Analysis of the 16S rRNA Gene of Anaplasma bovis in Cattle

ZHOU Zuo-yong1, 4, NIE Kui2, TANG Cheng3, HU Shi-jun1, ZHOU Rong-qiong1, ZHANG Ze4 *   

  1. 1.Department of Veterinary Medicine, Rongchang Campus, Southwest University, Chongqing 402460, China； 2. College of Animal Science and Technology, Southwest University, Chongqing 400715, China； 3. Chuanshan Animal Husbandry Bureau of Suining, Suining 629000, China； 4. Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400715, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-10-25 Published:2010-10-25

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Abstract: In the study，we cloned and sequenced the 16S rRNA gene of Anaplasma bovis isolated from Zhongxian in Chongqing. The 16S rRNA gene of A. bovis was 1 412 bp in length. The sequence was submitted to GenBank (accession number: FJ169957). The 16S rRNA gene of A. bovis (FJ169957) had highest homology (99.0%) with that by Kawahara published (AB211163). In addition, 5 homologous sequences from A. marginale, 4 from A. centrale, 4 from A. bovis, 4 from A. ovis and 3 from A. phagocytophilum available were downloaded from GenBank. Phylogenetic analyses on Anaplasma 16S rRNA sequences were performed. The results indicated that A. bovis has closer genetic relationship to A. phagocytophilum than that to A. marginale, A. centrale and A. ovis.