ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (10): 1225-1231.doi:

• 遗传繁育 • Previous Articles     Next Articles

Deletion Mutation of the Bovine SREBP-1c Gene of Jiaxian Red Cattle and Its Affecting on Growth Traits

HUANG Yong-zhen1, WANG Ju-qiang2, MA Gui-bian3, ZHANG En-ping1, HUAI Yong-tao1, MA Liang1, LEI Chu-zhao1, NIU Hui2, XIAO Jie2, CHEN Hong1*   

  1. 1. Key Laboratory of Agricultural Molecular Biology of Shanxi, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China; 2. Research Center of Cattle Engineering Technology of Henan, Zhengzhou 450003, China; 3. Pingdingshan Animal Husbandry Bureau, Pingdingshan 467000, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-10-25 Published:2010-10-25

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Abstract: The present study focused on screening the genetic variation within bovine SREBP-1c gene intron 7 and analyzing its effect on growth traits in 441 individuals belonging to Jiaxian Red cattle. In this study, the 84 bp deletion mutation of SREBP-1c gene was detected by using the DNA sequencing and agarose electrophoresis methods. The PCR products of SREBP-1c gene exhibited two genotypes WW and WD, and two alleles were revealed: W and D. Genotype WW was the predominant genotype and W was the predominant allele in the studied population. The frequencies of allelic W and D in Jiaxian Red cattle population were 0.865 1 and 0.134 9. Genetic indices (He, Ne and PIC) in this cattle population were presented low genetic diversity. The χ2test analysis demonstrated that the breed was in agreement with HardyWeinberg equilibrium (P>0.05). The association of the 84 bp deletion mutation of SREBP-1c gene with growth traits of Jiaxian Red cattle breed was analyzed. The animals with genotype WD had significantly higher body weight and heart girth than those with genotype WW (P<0.01 or P<0.05) at 12, 18 and 24 months. The animals with genotype WD had significantly greater body length than those with genotype WW (P<0.05) at 18 and 24 months. The individuals with genotype WD had significantly higher average daily gain than those with genotype WW (P<0.01) at 18 months. These results indicated that the growth stages and the selected traits should be comprehensively considered in the breeding practice of Jiaxian Red cattle. The genotype WD may have positive effect on growth traits. Therefore, these results suggest that the deletion mutation of bovine SREBP-1c gene may be a useful marker for growth traits in future marker-assisted selection programs in Jiaxian Red cattle.