ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2009, Vol. 40 ›› Issue (9): 1290-1296.doi:

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TNFα Stimulates Lipolysis in Differentiated Porcine Adipocytes and Wownregulated Expression of the Genes Related to Lipolysis

ZHENG Xueli 1,2, LI Yucheng 1, JU Dapeng 1, YANG Gongshe 1*

  

  1. 1. Laboratory of Animal Fat Deposition and Muscle Development, Northwest A&F University, Yangling 712100, China; 2. College of Forestry, Northwest A&F University, Yangling 712100, China
  • Received:2008-09-10 Revised:1900-01-01 Online:2009-09-24 Published:2009-09-24
  • Contact: YANG Gongshe

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Abstract:

To study the effect of tumor necrosis factor α(TNFα) on lipolysis in differentiated porcine adipocytes and its mechanism, the differentiated porcine adipocytes were treated with TNFα at the concentrations of 1, 10, 100 ng·mL1 for 24 hours and the concentration of 10 ng·mL1 for 0, 1, 6, 12, 24 and 48 hours , respectively. Commercial kits were used to measure the glycerol release; Semiquantitative RTPCR was used to detect the expression levels of ATGL,TGH2,HSL,LPL,MGL and perilipin A genes. The results showed that TNFα promoted the glycerol release in dose and time dependent manners(P<0.05) in differentiated porcine adipocytes，and downregulated the mRNA expression levels of ATGL, TGH2, HSL, LPL, MGL and perilipin A. These results indicated that TNFα could stimulate lipolysis by downregulating the expression level of the perilipin A which was thought to modulate the ATGL and HSL to the surface of the fat droplet.