Abstract:

To elucidate the effect of reactive oxygen species (ROS) on Sertoli Cell and provide some fundamental data for the study of mechanism of spermatogenesis, Sertoli Cell of piglets was chose to study the effect of ROS on microfilaments in Sertoli Cells. Sertoli Cells were treated with H2O2 and cytochalasins B, and vitamin E was used as an antioxidant. Cell viability, antioxygen enzymes activity and changes of activity of ERK1/2 (extracellular signalregulated kinase) were detected. The results showed as followed: H2O2 and cytochalasins B could increase the level of ROS and activity of ERK1/2 of cells, decrease antioxygen enzymes ability, and destroy the structure and distribution of microfilaments. H2O2 and cytochalasins B had synergistic effect on the level of ROS in Sertoli Cells, while Vitamin E decreased the effect of ROS on cell microfilaments. The results suggested that H2O2 resulted in the increase of ROS of Sertoli Cells by microfilamentindependence way and the decrease of antioxygen ability; the high level of ROS destroyed cell microfilaments through activating ERK.