Abstract: Aquaporin 7 (AQP7) is an newly discovered aquaglyceroprotein and responsible for the movement of glycerol In this study, we firstly obtained the sequence of intron 4 of the porcine AQP7 gene from Large White, Landrace and Meishan pig Sequence comparison revealed a G/A mutation which was detected by PCRPstIRFLP The G allele was predominant in the Large White and Landrace pigs, while there was higher A allele frequency in Meishan pigs Association analysis between the polymorphism and fat deposition traits was performed in 234 Large white×Meishan F2 pigs Significant associations of the genotype with fat deposition traits such as leaf fat weight (LFW), internal fat rate (IFR), backfat thickness (BT), fat percentage (FP) and ratio of lean meat versus fat meat (RLF) were found The individuals with GG genotype have lower fat deposition value than those with AA genotype and allele G was significantly associated with the decreasing of fat deposition