ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (3): 273-277.doi:

• 遗传繁育 • Previous Articles     Next Articles

Tissue Differential Expression of PPAR-γ in Bamei Pig

WANG Bo;WU Jiang-wei;YANG Gong-she   

  1. Laboratory of Animal Fat Deposition and Muscle Development,NorthwestA & F University,Yangling 712100 , China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-03-24 Published:2008-03-24

Abstract: Total RNA and protein were extracted from visceral adipose tissue, subcutaneous adipose tissue, skeletal muscle, kidney, liver, heart, lung and spleen of Bamei pig. The primers of PPAR-γ1 and PPAR-γ2 were designed and synthesized. The specific expression of PPAR-γ1 and PPAR-γ2 in different tissues were investigated by Semi-Quantitative RT-PCR and Western Blot. The results showed that the mRNA and protein of PPAR-γ1 were expressed in all abovementioned tissues, and highly expressed in visceral adipose tissue,subcutaneous adipose tissue and lower expressed in lung; PPAR-γ2 mRNA and protein were highly expressed in visceral adipose tissue, subcutaneous adipose tissue, spleen and heart, while remarkable lower in lung, kidney, liver and muscle. In conclusion, the results suggested that PPAR-γ might have various effects on different tissues of pig.