ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (11): 1493-1498.doi:

• 遗传繁育 • Previous Articles     Next Articles

Effect of Trichostatin A on in vitro Maturation of Porcine Oocyte and Development of Parthenogenetic Embryo

LIU Xiao1,2，PAN Deng-ke1*，CHEN Kou-kou1,3，FENG Chong1,3，ZHANG Wei-hong1,3，ZHENG Mao-en1,4，LONG Chuan1，FENG Shu-tang1，YANG Bo-hui2*   

  1. 1 The Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture,Institute of Animal Science，Chinese Academy of Agricultural Sciences，Beijing 100193,China； 2 Institute of Animal Science and Veterinary Pharmaceutics，Chinese Academy of Agricultural Sciences，Lanzhou 730050,China； 3Gansu Agricultural University， Lanzhou 730070,China； 4 Yunnan Agricultural University，Kunming 650201,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-11-24 Published:2008-11-24

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Abstract: Trichostatin A (TSA) is an inhibitor of histone deacetylase.It is reported that treatment of mouse somatic cell nuclear-transferred oocytes with TSA significantly increased the blastocyst rate.The present study was designed to examine the effect of TSA on the maturation of porcine oocytes and development of parthenogenetic embryos in vitro.We evaluated the concentration of TSA to oocyte maturation, the concentration of TSA to parthenogenetic embryo, treatment duration of TSA to parthenogenetic embryo in vitro. The maturation oocytes to the metaphase II (M II) stage cultured with 5 nmol/L TSA was not significantly different from the TSA-free treatment, meanwhile 5 nmol/L TSA treatment supported a higher cleavage and blastocyst development rate (P<0.05); The parthenogenetic embryo exposured in 50 nmol/L TSA supported a higher cleavage and blastocyst development rate (P<0.05).The parthenogenetic embryo exposured in 50 nmol/L TSA for 24 h supported a higher cleavage and blastocyst development rate than the others (P<0.05, 82.1±2.6% and 37.4 3.1% ).The data demonstrated that treatment of porcine oocytes and parthenogenetic embryos with TSA significantly improved the in vitro blastocyst production.5 nmol/L TSA treatment enhanced the oocytes maturation in vitro, and 50 nmol/L TSA-treatment for 24 h following oocyte activation resulted in more efficient development of parthenogenetic embryo.