ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (11): 1487-1492.doi:

• 遗传繁育 • Previous Articles     Next Articles

Effects of in vitro Developmental Competence of Bovine Somatic Cell Nuclear Transfer Embryos in Defined Culture Medium (IVD101 and G1/G2)

LI Rong1,3 ，LIU Ying3 ，ZHAO Xing-bo1，WANG Li-li2 ，WANG Hai-ping3，
DING Fang-rong3，LI Jing3，LI Song3，GAO Feng-lei3，DAI Yun-ping2*   

  1. 1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; 2. The State Key Laboratory of Agriobiotechnology, China Agricultural University, Beijing 100193, China; 3.Geneprint Biotechnology Co. LTD, Beijing 100193, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-11-24 Published:2008-11-24

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Abstract: The purpose of the study was to investigate in vitro development, quality and frozen tolerance of bovine somatic cell nuclear transfer (SCNT) embryos in two defined culture media (IVD101 and G1/G2). The SCNT embryos were cultured in three media: IVD101, G1/G2, and CR1aa+5%FBS (control group). The results indicated that the development rates of SCNT embryos cultured in IVD101 and G1/G2 were lower than that in CR1aa+5%FBS, but had no significant difference among three groups (41.2%±9.1%, 42.2%±10.8% and 48.0%±9.2%, respectively,P>0.05). The result of differential staining showed that the ratio of ICM/Total of blastocysts cultured in IVD101 and G1/G2 were lower than that in the group of CR1aa+5% FBS, whereas there was no significant difference among them (31.8%±10.5%, 29.5%±11.9% and 33.0%±14.8%, respectively, P>0.05). No significant difference was observed in the survival rate among three groups (84.8%, 80.4% and 77.3%, respectively, P>0.05). Our study demonstrated that defined cultured media (IVD101 and G1/G2) could support in vitro development of bovine SCNT embryos, and freezing tolerance of them were similar with the culture medium with FBS.