ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (10): 1426-1431.doi:

• 基础兽医 • Previous Articles     Next Articles

Expression of Recombinant Gallinacin-9 from Chicken in E.coli and Determination of Its Antimicrobial Activity

HAN Zong-xi2, MA De-ying1* ,LIU Sheng-wang2, LI Yi-jing3   

  1. 1.Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030,China; 2.Division of Avian Infectious Disease, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001,China; 3.College of Animal Medicine, Northeast Agricultural University, Harbin 150030,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-10-24 Published:2008-10-24

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Abstract: Gallinacin (Gal) -9 was cloned from tongue of chicks by RT-PCR and sequenced. The full length cDNA of Gal-9 consists of 201 bp, encoding 67 amino acids. The cDNA of chicken Gal-9 gene was sub-cloned into EcoR Ⅰ and Sal Ⅰ sites of pGEX-6p-1 vector to construct recombinant plasmid pGEX-Gal-9. The recombinant plasmid was transfected into E. coli BL21 and the transformed bacteria was induced with IPTG. It was demonstrated by SDSPAGE that a 32 kDa protein which was equal to chicken Gal-9 protein in molecular weight was expressed in E.coli BL21. The recombinant fusion protein was purified. The recombinant Gal-9 was expressed as inclusion bodies. The recombinant Gal-9 exhibit expected anti-E.coli and antiPathogenic streptococcus activity, as measured by inhibition zone assaying.