Abstract: In order to explore the regulation of the local-uterine cytokine IL-2, IL-4, TGF-β1 and TGF-β2 by selenium(Se) during early gestation of goats, the pregnant goats were grouped into experimental groups Ⅰ, Ⅱ, Ⅲ and control group. Group Ⅰ, Ⅱand Ⅲ were treated with sodium selenite respectively at doses of 0.3, 0.5 and 0.7 mg(Se)/kg by oral administration weekly for three times and control group was similarly treated with distilled water. Uterine samples were collected to determine the content of IL-2,IL-4,TGF-β1 and TGF-β2 by ELISA. The results showed that (1)There were no differences at the levels of all the tested cytokine between two uterine horns of each goat during early gestation. The IL-2 levels in fluid of two uterine horns increased during 15-18 d after pregnancy and began to decrease on day 19 after pregnancy, and IL-4 remained in a continuously high level during 15-19 d after pregnancy. The TGF-β₁ had irregular high level in the same period and TGF-β₂ increased primely and then remained at a high level. (2) Administration of 0.3, 0.5 and 0.7 mg/kg selenium could significantly decrease the content of IL-2 in uterine fluid during 16-18 d after pregnancy and significantly increase the level of IL-4 in uterine fluid in a dose-dependent manner. (3)Goats of 0.5 and 0.7 mg/kg groups showed significantly high level of TGF-β₁ in uterine fluid during 16-18 d after pregnancy, and presented high level of TGF-β₂ in uterine fluid during 16-17 d after pregnancy but no significant effect on TGF-β₂ secretion on day 18 after pregnancy. These results indicated that selenium has a regulatory function on secretion of the local-uterine IL-2, IL-4, TGF-β₁ and TGF-β₂ during early pregnancy in goats.