Abstract: Single nucleotide polymorphisms (SNPs) were detected by RTPCR amplification and sequencing on the EST (GenBank accession No. BM445313)which was expressed in porcine muscle. Two new SNPs were found and located in the 3′UTR region of Myopalladin gene by homologous comparative analysis in the nonredundant sequence database of NCBI through BLAST search. One of the SNPs was genotyped by RFLP with restriction enzyme Csp6I and used for association analysis with carcass traits in the SCAU-LL F₂ pig resource population. The results showed that the average carcass lean meat percentage of CT and CC genotype were 46% and 45% respectively, and the average carcass skin & fat percentage of the two genotype were 42% and 44% respectively, these differences were significant (P<0.05). Therefore, it could be seen that porcine Myopalladin genotypes had significant influence on carcass traits, and might be considered as a potential genetic marker for selection.