ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (7): 685-693.doi:

• 预防兽医 • Previous Articles     Next Articles

Detection of Classical Swine Fever Virus in Experimentally Infected Pigs Using a Newly Developed Real-time Fluorescent Quantitative RT-PCR

ZHAO Jian-jun;CHENG Dan;LI Na;SHI Zi-xue;SUN Yuan;ZHAO He-ping;ZHU Qing-hu;TU Chang-chun;QIU Hua-ji   

  1. 1.National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001,China; 2.College of Veterinary Sciences, Xinjiang Agricultural University, Urumuqi 830052,China; 3.Institute of Vetevinavy Sciences, Academy of Military Medical Sciences, Changchun 130062,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-07-25 Published:2007-07-25

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Abstract: A real-time fluorescent quantitative RT-PCR assay for detecting classical swine fever virus (CSFV) was established and evaluated in experimentally infected pigs.The sensitivity of the assay was 10 copies/μL viral RNA, with an agreement of 94.7% with RT-nested PCR in detecting 152 different samples. The assay was used to detect CSFV in the whole blood samples of the pigs infected with 106 TCID50 Shimen strain and contact pigs. The results showed that there was an excellent correlation between clinical symptoms and viral RNA loads in the blood.Viral RNA can be detected in the blood samples of contact pigs 3 to 4 days prior to the onset of clinical signs.