ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (7): 694-699.doi:

• 预防兽医 • Previous Articles     Next Articles

Construction of Co-expressing Plasmids of AgB from Taenia Solium with Porcine CD58 or IFN-γ Gene and Expression in BHK-21 Cells

HOU Jun-ling;JING Zhi-zhong;ZHENG Ya-dong;HU Zhi-min; LUO Xue-nong;MENG Xue-lian;DOU Yong-xi;LIU Jun-long;CAI Xue-peng   

  1. Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-07-25 Published:2007-07-25

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Abstract: Cysticercosis is an important zoonosis.The development of efficient, safety and cheap vaccines is one of contemporary major directions. In this study, AgB, CD58 and IFN-γ gene were amplified from the former constructed recombinant plasmids named pGEM-TEasy-AgB, pGEM-TEasy-CD58 and pGEM-TEasy-IFN-γ by PCR and cloned into pBudCE4.1.The acquired recombinant plasmids were transfected into BHK-21 cell with liposome. 48 hours after transfection, the expression of AgB, CD58 and IFN-γ were tested by indirect immunofluorescence assay (IFA) or direct immunofluorescence assay.The results showed that AgB, CD58 and IFN-γ were successfully expressed in BHK-21 cells, providing a solid basis for development of DNA vaccine against cysticercosis