ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2007, Vol. 38 ›› Issue (12): 1389-1393.doi:

• 研究简报 • Previous Articles     Next Articles

Production of Transgenic Embryos Expressing Green Fluorescent Protein by Nuclear Transfer of Preadipocytes in Pig

PAN Deng-ke;WAN Rong;LIU Ji;MU Yu-lian;ZHANG Li;LI Kui;FENG Shu-tang   

  1. 1. The Key Laboratory of Domestic Animal Genetic Resources and Germplasm Innovation of CAAS,Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China; 2.State Key Laboratory of Animal Nutrition,College of Animal Science and Technology, China Agricultural University, Beijing 100094, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-12-25 Published:2007-12-25

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Abstract: Primary culture of porcine preadipocytes was established using fat tissue from an adult pig. The preadipocytes to re-differentiate induction into mature adipocytes was confirmed by oil red O staining. A porcine preadipocyte (PreA) lines were transfected with pEGFP-N1 plasmid DNA by lipofectinmediated way. GFPPreA clones were obtained by G418 selection. PreA, fetal fibroblast (FF), and GFP-PreA were used as nuclear donors for somatic cell nuclear transfer in pig. The results were as follows: For the blastocyst rate of cloned embryos, no significant difference was observed between PreA and FF (8.8% vs 8.3%, P > 0.05). Blastocyst rate of reconstructed with GFP transfected PreA decreased in comparison to those derived from nontransgenic PreA.（3.7% vs. 8.8%，P > 0.05）。The results indicate that GFP transfected PreA can produce procine transgenic blastocytes. In spite of low blastocyst rate, cloning pigs from preadipocyte is possible.