ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2005, Vol. 36 ›› Issue (9): 969-973.doi:

• 研究简报 • Previous Articles     Next Articles

The Detection of Actinobacillus pleuropneumoniae by Real-time PCR

LI Shu-qing;YI Jian-ping;LI Jian;HU Yong-qiang;WANG Qiao-quan;CHEN Zhi-fei;ZHOU Xiao-hua;PAN Xiao-zhong;LUO Man-lin;CHEN Min
  

  1. 1. Shanghai Export and Import Inspection and Quarantine Bureau, Shanghai 200135,China；2. South China Agricultural University, Guangzhou 510642,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-09-25 Published:2005-09-25

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Abstract: Primers FP/ RP and TaqMan-MGB Probe were designed from sequence of apxIVA gene specific to all serotypes of Actinobacillus pleuropneumoniae. A real-time PCR method was developed for detecting Actinobacillus pleuropneumoniae, which can amplify all strains of APP. The sensitivity is 12fg DNA or 5.1 CFU bacteria. This method can be directly used for detection of APP without DNA extraction.