Abstract: The mature region DNA for Myostatin in Jinhua pig, Xianju chicken, Shao duck were cloned and sequenced. Comparing with the corresponding species’ sequence that reported in GenBank, we found that there had one base mutation（T²⁶¹→A²⁶¹）in Jinhua pig and two base mutation（T²¹⁸→C²¹⁸，C²¹⁹→T²¹⁹）in Xianju chicken. The duck’s sequence has not been reported in GenBank. We analyzed the homology of the sequences in all species. The results showed that Myostatin has highly conservation of heredity. The homologies in DNA level are all above 81% (except fish). We also discussed the possible Myostatin signaling pathway and the evolution relationship between all species.