ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2005, Vol. 36 ›› Issue (12): 1354-1357.doi:

• 研究简报 • Previous Articles     Next Articles

Study of Differentiation of Chicken Primordial Germ Cells into Neuronlike Cells

CAI Lin-lin;QIN Jie;LI Bi-chun; XIAO Xiao-jun;CHEN Guo-hong;WU Xin-sheng;WU Sheng-long   

  1. 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China；2.College of Life Science, Huzhou University, Huzhou 313000, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2005-12-25 Published:2005-12-25

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Abstract: Primordial germ cells (PGCs) isolated from gonads of stage 19 by Ficoll density gradient centrifugation were cultured in DMEM medium in vitro. Then the second passage PGCs were induced to differentiate into neuron-like cells by different induction media. Specific markers and structures were detected by histochemistry method. The results showed that induction medium RA and IBMX were the best ways to induce PGCs into neuron-like cells, and almost 80%-85% cells exhibited typical neuron-like phenotype after induction. Special Nissl body was found by histochemistry. The effect of induction medium RA+IBMS,RA+BHA+DMSO and RA+BHA+DMSO+IBMX were better than that of induction medium BHA+DMSO.Under the same induction medium, about 80%-85% neuronlike cells could be detected no matter what PGCs were cultured 24 h, 48h or 72 h in vitro.