K亚群禽白血病病毒5'LTR序列及启动活性分析

doi:10.11843/j.issn.0366-6964.2018.04.012

Abstract

Abstract:

In this study, we investigated the proliferation of avian leukosis virus subgroup K (ALV-K) and the activity of LTR. An ALV-K strain was used to infect DF-1 cells and the LTR was cloned into pGL3-Basic vector. The replication ability of the virus on ALV susceptible cell DF-1 was evaluated by ALV p27 antigen ELISA, and the promoter activities of the LTR of ALV-K, ALV-E and ALV-J strains were measured using a Dual-Glo Luciferase Assay System. The results showed that promoter activity of ALV-K LTR was significantly lower than those of other exogenous ALV in DF-1, CEF and 293T cells. The relatively poorer replication ability of ALV-K may be related to its weak promoter activity of the LTR.

CLC Number:

S852.659.3

ZHAO Zi-jun, RAO Ming-zhang, CHEN Jian, ZHANG Jie, YUAN Li-xia, LIAO Ming, CAO Wei-sheng. The Research of 5'LTR Promoter Activity of Avian Leukosis Virus Subgroup K[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(4): 754-760.

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References

[1] 杨玉莹. J亚群禽白血病病毒研究进展[J]. 中国病毒学, 2003, 18(1):93-97.
YANG Y Y. Advance in Avian leukosis virus subgroup J[J]. Virologica Sinica, 2003, 18(1):93-97. (in Chinese)
[2] PAYNE L N. Developments in avian[J]. Leukemia, 1999, 6(3):150-152.
[3] 王鑫, 赵鹏, 崔治中. 我国地方品种鸡分离到的一个禽白血病病毒新亚群的鉴定[J]. 病毒学报, 2012, 28(6):609-614.
WANG X, ZHAO P, CUI Z Z. Identification of a new subgroup of avian leukosis virus isolated from Chinese indigenous chicken breeds[J]. Chinese Journal of Virology, 2012, 28(6):609-614. (in Chinese)
[4] CUI N, SU S, CHEN Z M, et al. Genomic sequence analysis and biological characteristics of a rescued clone of avian leukosis virus strain JS11C1, isolated from indigenous chickens[J]. J Gen Virol, 2014, 95(Pt 11):2512-2522.
[5] 郝建勇, 秦建如, 邱倩倩, 等. 地方鸡种K亚群禽白血病病毒的分离及遗传鉴定[C]//中国畜牧兽医学会. 中国畜牧兽医学会2014年学术年会论文集. 广州:中国畜牧兽医学会, 2014.
HAO J Y, QIN J R, QIU Q Q, et al. Identification and evolution analysis of ALV-K isolated from local Chinese chicken[C]. Guangzhou:Chinese Association of Animal Science and Veterinary Medicine, 2014. (in Chinese)
[6] LI X J, LIN W C, CHANG S, et al. Isolation, identification and evolution analysis of a novel subgroup of avian leukosis virus isolated from a local Chinese yellow broiler in South China[J]. Arch Virol, 2016, 161(10):2717-2725.
[7] SHAO H X, WANG L, SANG J J, et al. Novel avian leukosis viruses from domestic chicken breeds in mainland China[J]. Arch Virol, 2017, 162(7):2073-2076.
[8] CHANG S W, HSU M F, WANG C H. Gene detection, virus isolation, and sequence analysis of avian leukosis viruses in Taiwan country chickens[J]. Avian Dis, 2013, 57(2):172-177.
[9] 张青婵. A亚群禽白血病病毒不同分离株的基因组和生物学特性比较[D]. 泰安:山东农业大学, 2010.
ZHANG Q C. Comparison of genome and biological characteristic of different subgroup A avian leukosis virus strains[D]. Tai'an:Shandong Agricultural University, 2010. (in Chinese)
[10] 高玉龙, 邵华斌, 罗青平, 等. 2009年我国部分地区禽白血病分子流行病学调查[J]. 中国预防兽医学报, 2010, 32(1):32-35, 43.
GAO Y L, SHAO H B, LUO Q P, et al. Molecular epidemiology of avian leukosis virus isolates from some regions of China in 2009[J]. Chinese Journal of Preventive Veterinary Medicine, 2010, 32(1):32-35, 43. (in Chinese)
[11] 王帅. 地方品系鸡白血病流行病学调查与ALV p27基因的表达分析[D]. 泰安:山东农业大学, 2013.
WANG S. Epidemiological investigation of avian leukosis in local strains chicken and expression analysis of ALV p27 gene[D]. Tai'an:Shandong Agricultural University, 2013. (in Chinese)
[12] 冯敏, 谭利强, 代曼曼, 等. 种禽场A亚群禽白血病病原学调查及分离株遗传进化分析[J]. 华南农业大学学报, 2014, 35(4):11-15.
FENG M, TAN L Q, DAI M M, et al. Etiology surveys and phylogenetic analyses of avian leukosis virus subgroup A isolated from breeder chickens farms[J]. Journal of South China Agricultural University, 2014, 35(4):11-15. (in Chinese)
[13] 郝建勇. 黄羽肉鸡配套系核心群AL净化方案的研究[D]. 广州:华南农业大学, 2015.
HAO J Y. Eradication protocol study of Avian Leukosis in Yellow broiler chickens[D]. Guangzhou:South China Agricultural University, 2015. (in Chinese)
[14] 秦立廷, 高玉龙, 潘伟, 等. 我国部分地区蛋鸡群ALV-J及与REV、MDV、CAV混合感染检测[J]. 中国预防兽医学报, 2010, 32(2):90-93.
QIN L T, GAO Y L, PAN W, et al. Investigation of co-infection of ALV-J with REV, MDV, CAV in layer chicken flocks in some regions of China[J]. Chinese Journal of Preventive Veterinary Medicine, 2010, 32(2):90-93. (in Chinese)
[15] 张振杰, 刘绍琼, 王波, 等. 地方品种皖南黄肉种鸡ALV-J与REV的共感染及其分子变异分析[J]. 中国农业科学, 2011, 44(11):2379-2386.
ZHANG Z J, LIU S Q, WANG B, et al. Identification of co-infection of ALV-J and REV and molecular characterization of the two viruses isolated from Wannan yellow feather broilers[J]. Scientia Agricultura Sinica, 2011, 44(11):2379-2386. (in Chinese)
[16] 李久庆, 刘强, 郭雷, 等. 一株新亚群禽白血病病毒全基因组序列分析[J]. 畜牧兽医学报, 2017, 48(9):1718-1723.
LI J Q, LIU Q, GUO L, et al. Genomic sequence analysis of a new subgroup of avian leukosis virus[J]. Acta Veterinaria et Zootechnica Sinica, 2017, 48(9):1718-1723. (in Chinese)
[17] BROWN D W, ROBINSON H L. Influence of env and long terminal repeat sequences on the tissue tropism of avian leukosis viruses[J]. J Virol, 1988, 62(12):4828-4831.
[18] 张贺楠, 齐岩, 史伟伟, 等. 不同病变型J亚群禽白血病病毒LTR启动子序列分析及活性比较[J]. 病毒学报, 2010, 26(5):402-406.
ZHANG H N, QI Y, SHI W W, et al. Sequence and promoter efficacy analysis of avian leukosis virus subgroup J strains of different pathotypes[J]. Chinese Journal of Virology, 2010, 26(5):402-406. (in Chinese)
[19] 冯少珍, 李娇, 吴晓婵, 等. 不同亚群禽白血病病毒5'LTR序列及启动子活性分析[J]. 中国畜牧兽医, 2011, 38(8):125-131.
FENG S Z, LI J, WU X C, et al. Sequence and promoter activity analysis of 5'LTR from different subgroups of avian leukosis viruses[J]. China Animal Husbandry & Veterinary Medicine, 2011, 38(8):125-131. (in Chinese)

The Research of 5'LTR Promoter Activity of Avian Leukosis Virus Subgroup K

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