（一）-HCA对鸡胚原代肝细胞增殖和线粒体功能的影响

doi:10.11843/j.issn.0366-6964.2017.05.020

Abstract

Abstract:

The present study aimed to investigate the effects of (一)-Hydroxycitric acid (HCA) on cells proliferation, mitochondrial ultrastructure and function in primary chicken hepatocytes. The cells were treated with 0, 1, 10 and 50 μmol·L^-1 (一)-HCA and then detected the factors related with cells proliferation and mitochondria function. The results showed that (一)-HCA treatment promoted the cell proliferation in primary chicken hepatocytes. Ten μmol·L^-1 (一)-HCA treatment significantly increased the S phase and G2/M phase cell population (P<0.01), meanwhile it increased the cyclin mRNA expression level when compared to control group. No significantly changes were observed on the mitochondrial morphology, quantity and membrane potential in primary chicken hepatocytes following 1-50 μmol·L^-1 (一)-HCA treatment (P>0.05), while (一)-HCA treatment significantly increased the activities of succinate dehydrogenase and citrate synthase in primary chicken hepatocytes (P<0.05). These results indicated that (一)-HCA treatment promote the primary chicken hepatocytes proliferation by increasing cyclin mRNA expression, whereas it increase the energy metabolism by enhancing the activities of succinate dehydrogenase and citrate synthase in primary chicken hepatocytes.

CLC Number:

S852.23

LI Long-long, PENG Meng-ling, WANG Dian, YU Lei, GE Chong-yang, MA Hai-tian. The Effect of (一)-Hydroxycitric Acid on Proliferation and Mitochondria Function in Cultured Primary Chicken Hepatocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 945-953.

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The Effect of (一)-Hydroxycitric Acid on Proliferation and Mitochondria Function in Cultured Primary Chicken Hepatocytes

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