不同尾型绵羊全基因组选择信号检测

doi:10.11843/j.issn.0366-6964.2015.10.004

Abstract

Abstract:

This experiment was conducted to study genetic differentiation between 2 sheep populations with distinct tail types by genome-wide detection of selection signatures and to search the candidate genes related to important traits of sheep.Based on the Illumina Ovine SNP 50K Breadchip genotyping data of Mongolian sheep and Tibetan sheep，population differentiation index FST was adopted to detect the selection signatures，and to found the genes located in selection signature regions.Based on the tail adipose tissue of Hulun Buir sheep(fat-tailed sheep，include large fat-tailed sheep and small fat-tailed sheep) and Tibetan sheep(thin-tailed sheep)，the relative expression study was adopted to explore the difference of gene relative expression level of PPARG and PDGFD，which were related to fat metabolism.This result showed that：(1) 465 selected SNPs were found，448 candidate genes were detected by gene annotation． And 50 genes related to lipid metabolism were selected from these candidate genes，the analysis of GO showed that these genes were related to lipid biosynthetic process，phospholipid metabolic process and lipid binding.In addition，4 genes were found，PPARG，RXRG，SLC27A2 and ACSL6，and enriched into the PPAR signaling pathway.(2)The gene relative expression levels of PPARG and PDGFD from Hulun Buir sheep was significantly higher than that of Tibetan sheep(P<0.01)， between fat-tailed and thin-tailed sheep，the gene relative expression level of PPARG from lager fat-tailed Hulun Buir sheep was significantly higher than that of small fat-tailed Hulun Buir sheep(P<0.05)，but the gene relative expression level of PDGFD had no significant difference between lager fat-tailed and small fat-tailed Hulun Buir sheep.F_ST could be used to detect the genes with selection signatures and some of the candidate genes were related to important traits in sheep.PPARG and PDGFD genes were proved to be closely related to tail fat deposition through the relative expression studies，these genes could be used as candidate genes for different tailed sheep breeding.This study can provide theoretical references for sheep breeding or breed improvement.

CLC Number:

S826.2

LIU Zhen，WANG Hui-hua，LIU Rui-zao，WU Ming-ming，ZHANG Shu-zhen，ZHANG Li，ZHAO Fu-ping，DU Li-xin，WEI Cai-hong. Genome-wide Detection of Selection Signatures of Distinct Tail Types in Sheep Populations[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(10): 1721-1732.

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Genome-wide Detection of Selection Signatures of Distinct Tail Types in Sheep Populations

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