基于线粒体cox1和Cyt b基因对四川地区多头带绦虫的种群遗传多样性研究

doi:10.11843/j.issn.0366-6964.2014.04.018

Abstract

Abstract:

To study the population genetic diversity of T.multiceps isolated from Sichuan and the phylogentic relationships with other taenia species，the complete sequences of mitochondrial Cytochrome c oxidase subunit 1 (cox1) and Cytochrome b (Cyt b) genes of 20 Coenurus collected from goats (11 from brain，and 9 from intramuscular) in Sichuan were analyzed and phylogentic trees were reconstructed.The results showed that the complete lengths of mitochondrial genes sequenced in this study were 1 623 bp for cox1 gene and 1 068 bp for Cyt b gene.Variable sites were 59 and 9，respectively.The sequence divergence between the cox1 sequences of Coenurus isolated from Sichuan ranged from 0.1% to 2.5%，while differences in Cyt b sequences ranged from 0.1% to 0.7%.Seventeen haplotypes and 6 haplotypes were detected in cox1 and Cyt b，with the global haplotype diversity being 0.968 ± 0.033 (cox1) and 0.737 ± 0.068 (Cyt b)，the nucleotide diversities were 0.005 98 ± 0.001 33 for cox1 and 0.003 06 ± 0.000 79 for Cyt b，and the average genetic distances were 0.006 for cox1 and 0.003 for Cyt b，respectively.The phylogenetic trees inferred from both cox1 and Cyt b showed that all 20 Coenurus isolated from Sichuan were the species T.multiceps.A closer relationship was found between Sichuan isolates and Iran and Turkey isolates，and it was closer than that between Sichuan and Gansu，Guangzhou and Italy isolates.The results indicated that cox1 gene and Cyt b gene were suitable markers for detecting genetic diversity of T.multiceps，and the diversity of T.multiceps based on Cyt b gene was lower than cox1 gene.

CLC Number:

S852.734

HAO Gui-ying，YANG Ying-dong，ZHOU Ying-zi,YANG Guang-you. Population Genetic Diversity of Taenia multiceps Isolated from Sichuan Based on Mitochondrial cox1 and Cyt b Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 631-638.

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Population Genetic Diversity of Taenia multiceps Isolated from Sichuan Based on Mitochondrial cox1 and Cyt b Gene

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