奶牛临床型乳房炎与健康乳腺组织蛋白表达图谱的初步分析

畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (11): 1248-1252.

奶牛临床型乳房炎与健康乳腺组织蛋白表达图谱的初步分析

杨永新;赵兴绪;张进隆;陶金忠;张勇

1甘肃农业大学动物医学院，兰州 730070；2安徽农科院畜牧兽医研究所，合肥 230031；3甘肃畜牧工程职业技术学院兽医系，武威 733006

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-11-25 发布日期:2007-11-25

Proteome Maps of Mammary Tissues in Healthy Dairy Cows and Those with Clinical Mastitis

YANG Yong-xin;ZHAO Xing-xu;ZHANG Jin-long;TAO Jin-zhong;ZHANG Yong

1College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070 , China；2Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031,China;3Department of Veterinary, Gansu Vocational College of Animal Husbandry and Engineering, Wuwei 733006,China

Received:1900-01-01 Revised:1900-01-01 Online:2007-11-25 Published:2007-11-25

摘要/Abstract

摘要： 本研究以二维电泳技术构建了临床型乳房炎奶牛和健康奶牛的乳腺组织全蛋白表达谱，运用PDQuest7.4软件进行差异分析。结果显示：健康奶牛的乳腺组织蛋白表达图谱可检测到（480±27）个蛋白点，临床型乳房炎奶牛可检测到（453±31）个蛋白点，二者存在17个差异表达的蛋白斑点，6个蛋白斑点在临床型乳房炎奶牛组织中呈量度，4个呈质变。表明临床型乳房炎奶牛和健康奶牛的乳腺组织蛋白存在差异表达的蛋白斑点，本研究为发现新的乳房炎调控分子和潜在的医疗目标蛋白奠定了基础。

关键词: 奶牛, 乳腺组织, 乳腺炎, 蛋白质组学, 二维电泳

Abstract: In the mammary glands suffered from invading pathogens that produced and released inflammatory mediators might be accompanied by changes in protein expression profiles. This project presents the protein changes of the mammary gland from healthy dairy cows and clinical mastitic cows that were investigated using two-dimensional gel electrophoresis (2-DE). Acquired gel maps were matched and analyzed using PDQuest 7.4 image analysis software. The results showed that the protein profiles in healthy dairy cows and those with clinical mastitis were shown to be very similar, 480±27 and 453±31 protein spots were statistically detected, respectively. Take "more or less" as the determination criterion, 17 protein spots were detected to be differentially expressed changes in the stain density of threefold or more. That is, 10 protein spots were up or downregulated proteins in clinical mastitic cows, 7 protein spots were unique in healthy dairy cows or clinical mastitic cows. The results indicated significant difference in expression protein between healthy dairy cows and those with clinical mastitis, and may provide valuable information to find new regulation markers of mastitis and potential protein targets for treatment.

Key words: dairy cow, mammary gland, mastitis, proteomics, twodimensional electrophoresis

杨永新;赵兴绪;张进隆;陶金忠;张勇. 奶牛临床型乳房炎与健康乳腺组织蛋白表达图谱的初步分析[J]. 畜牧兽医学报, 2007, 38(11): 1248-1252.

YANG Yong-xin;ZHAO Xing-xu;ZHANG Jin-long;TAO Jin-zhong;ZHANG Yong. Proteome Maps of Mammary Tissues in Healthy Dairy Cows and Those with Clinical Mastitis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2007, 38(11): 1248-1252.

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