关键词: 结肠弯曲杆菌, 耐药性, 多位点序列分型, 基因型

Abstract:

To determine the phenotypic and genotypic resistance of campylobacter coli(C.coli) isolates from poultry meat, 54 C.coli isolates from retail poultry meat were phenotyped for ciprofloxacin(CIP),erythromycin(ERY),doxycycline(DOX) and gentamicin(GEN) by agar dilution method, and were genotyped by multilocus sequence typing (MLST). Within the 54 C.coli isolates,resistance only to CIP,ERY and DOX were respectively 16.7% ,16.7% and 42.6%, 18.5% of the isolates were resistant to two or three antimicrobial agents,whereas none of the isolates were resistant to GEN.MLST analysis of 54 isolates identified 38 alleles and 26 STs, including 1 new allele and 8 new STs.26 STs revealed ST828 clonal complexes, ST1150 clonal complexes and 5 STs not assigned clonal complexes in the international databases at http://pubmlst.org/campylobacter/. ST828 clonal complexs were predominant(83.3%,45 isolates). MLST did not group the isolates by their resistance and susceptibility to these four antimicrobial agents. These results indicated that resistance to ciprofloxacin, erythromycin and doxycycline were found in C. coli from poultry meat, and ST828 clonal complex was mainly clonal complex in poultry meat. There was no linear relationships between the resistance and susceptibility to these four antimicrobial agent and ST clonal or ST clonal complexs.

Key words: C.coli, antibiotic resistance, MLST, genotype