关键词: 山羊, ADD1, 克隆, 序列分析

Abstract: Extracted total DNAs from Xiangdong Black goat, the caprine ADD1(Adipocyte Determination and Differentiation factor-1) gene were amplified by polymerase chain reaction , using three pairs of primers, and then were coloned and sequenced. Sequencing results of the PCR-derived fragments showed that the amplified fragments contained the exon 2-8 and intron 2-7 of caprine ADD1 gene. We sent the sequences to GenBank and obtained two accession number : DQ480338，DQ487874; Blasted the coding sequences(exon 2-8) with the bovine and human being, the nucleotide identities were 96.14%,83.82%, respectively, even the amino acid identities was 97.9％ comparing with the bovine ; furthermore , comparing the sequencing results of different primers ,we find a base conversion（C→T）and a base insert or absence（G/-） located on the 42th of exon 6 and the 82th of intron 6, respectively, and the former base conversion which was silent mutation made no AA transformation.

Key words: goat, ADD1, cloning, sequence analysis