关键词: 猪带绦虫, TSOL18, 45W-4BX, 疫苗, 保护效果

Abstract: Genes 45W-4B and TSOL18 were amplified by RT-PCR. The truncated 45W-4B named 45W-4BX was acquired by PCR.45W-4BX and TSOL18 were subcloned into pGEX-4T-1， respectively. Soluble 45W-4BX and TSOL18 in the form of inclusive bodies were detected by SDS-PAGE, Western blot and the results indicated that they could be recognized by sera from cysticercosis patients. Purified antigens 45W-4BX, TSOL18 and GST and cysticercus cellulosae coarse extract were used to immunize pigs, which were experimentally challenged with 25 000 eggs of Taenia solium 28 days postimmunization. Data suggested that antibody level would start to raise at 15 days and reach peak at 45 days postimmunization. Reduction in the number of cysticerci in the groups immunized with recombinant proteins were above 88%. Level of protection of groups vaccinated with TSOL18 and 45W-4BX did not show significant difference in comparison with the cysticercus cellulosae coarse extract. Recombinant antigens TSOL18 and 45W-4BX were primarily elucidated and their protective efficacy was confirmed.

Key words: Taenia solium, TSOL18, 45W-4BX, vaccine, protective efficacy