黄芪甲苷对微血管自律性舒缩活动及大鼠心肌微血管内皮细胞NO、ET-1的影响

doi:10.11843/j.issn.0366-6964.2019.04.021

摘要/Abstract

摘要：

为探讨黄芪甲苷（Ast）对人合谷穴区皮内微血管自律性舒缩活动振幅及大鼠心肌微血管内皮细胞（RMMECs）NO、ET-1分泌量的影响，利用激光多普勒血流测定仪结合离子导入仪检测微血管自律性舒缩活动的振幅；利用ELISA方法分别检测5、10、25 μg·mL^-1Ast孵育3、6、9、12 h对RMMECs分泌NO和ET-1的影响；利用Real-Time PCR检测10 μg·mL^-1Ast作用RMMECs后ET-1和NO基因转录量的变化。结果显示，人合谷穴区皮下导入Ast后，微血管舒缩振幅提高。与对照组比较，低、中、高浓度的Ast均能促进RMMECs分泌NO、ET-1，以及NO/ET-1的比值升高。10 μg·mL^-1 Ast与RMMECs作用3 h时，NO/ET-1值最高，NO、ET-1基因转录水平升高，与分泌水平的结果一致。25 μg·mL^-1 Ast与RMMECs作用3 h时，其NO/ET-1值略低于10 μg·mL^-1 Ast组，但其比率可相对稳定地维持在较高水平。结果表明，中药成分Ast能提高微血管舒缩振幅，其机制可能是通过调节RMMECs释放NO和ET-1在循环系统中的动态平衡。

关键词: 微血管自律运动, Ast, NO, ET-1, RMMECs

Abstract:

The study was conducted to investigate the effects of astragaloside (Ast) on microvascular vasomotion at Hegu acupoint, nitric oxide and endothelin-1 released by rat myocardial microvascular endothelial cells (RMMECs). Laser Doppler flowmetry combined with iontophoresis was used to detect the amplitude of microvascular vasomotion. ELISA method was used to detect the levels of NO and ET-1 released by RMMECs incubated with different doses of Ast for 3, 6, 9 and 12 hours. Real-Time PCR was used to detect the gene transcription of NO and ET-1 in RMMECs treated with 10 μg·mL^-1Ast. The results were as follows:After introduction of Ast, the amplitude of microvascular vasomotion at Hegu acupoint was improved. Compared with control group (RMMECs), the levels of NO and ET-1 were improved, NO/ET-1 value was increased by Ast in Ast incubated RMMECs. When RMMECs were incubated with 10 μg·mL^-1 Ast for 3 hours, the NO/ET-1 value was the highest, and the gene transcription levels of NO and ET-1 were improved, which were consistent with the results of secretion level. When 25 μg·mL^-1 Ast incubated RMMECs for 3 hours, its NO/ET-1 value was slightly lower than that of 10 μg·mL^-1 Ast group, but its ratio could be maintained at a relatively high level. The results showed that Ast could increase the amplitude of microvascular vasomotion, and its mechanism might be through regulating the dynamic balance of NO and ET-1 released by RMMECs in circulatory system.

Key words: microvascular vasomotion, astragaloside, NO, ET-1, RMMECs

中图分类号:

Q251

李春晓, 刘小瑜, 王鑫, 王月明, 韦东来, 李秋月, 贺尚文, 穆祥, 董虹. 黄芪甲苷对微血管自律性舒缩活动及大鼠心肌微血管内皮细胞NO、ET-1的影响[J]. 畜牧兽医学报, 2019, 50(4): 879-886.

LI Chunxiao, LIU Xiaoyu, WANG Xin, WANG Yueming, WEI Donglai, LI Qiuyue, HE Shangwen, MU Xiang, DONG Hong. Effect of Astragaloside on Microvascular Vasomotion and Nitric Oxide, Endothelin-1 Secretion from Rat Myocardia Microvascular Endothelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 879-886.

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