人参皂苷Rb1对微血管自律性舒缩活动及大鼠心肌微血管内皮细胞内钙瞬时变化的影响

doi:10.11843/j.issn.0366-6964.2015.01.020

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (1): 156-162.doi: 10.11843/j.issn.0366-6964.2015.01.020

人参皂苷Rb1对微血管自律性舒缩活动及大鼠心肌微血管内皮细胞内钙瞬时变化的影响

王鑫¹，冯波²，孙向婉¹，李平丽¹，董虹^1*，穆祥^1*

(1.北京农学院兽医学（中医药）北京市重点实验室，北京 102206；2.中国农业大学动物医学院，北京 100193)

收稿日期:2014-10-28 出版日期:2015-01-23 发布日期:2015-01-23
通讯作者: 董虹，副教授，Tel：010-80799480，E-mail：donghong523@163.com; 穆祥，教授，Tel：010-80789515，E-mail：muxiang1109@sina.com
作者简介:王鑫（1992-），男，江西靖安人，硕士生，主要从事中兽医药及疾病防控方面研究，E-mail：williamxin0217@163.com
基金资助:
国家自然科学基金（31101850）；北京市教委科技发展计划重点项目(KZ201110020021)；北京市教委科技面上项目（KM201210020008）；北京市自然基金（6132007）；北京市科技新星（Z141105001814041）；2014年北京农学院学位与研究生教育改革与发展项目（2014YJS057）

Effect of Ginsenoside Rb1 Induce Microvascular Vasomotion and the Effect on Sensitive Threshold of Rat Myocardial Microvascular Endothelial Cells

WANG Xin¹，FENG Bo²，SUN Xiang-wan¹，LI Ping-li¹，DONG Hong^1*，MU Xiang^1*

（1.Beijing Key Laboratory of Traditional Chinese Veterinary Medicine，Beijing University of Agriculture，Beijing 102206，China;2.College of Veterinary Medicine，China Agricultural University，Beijing 100193，China)

Received:2014-10-28 Online:2015-01-23 Published:2015-01-23

摘要/Abstract

摘要：

探讨人参皂苷Rb1（GSRb1）对人合谷穴区皮内微血管自律性舒缩活动振幅及大鼠心肌微血管内皮细胞（RMMECs）内游离钙离子瞬时变化的影响。利用激光多普勒血流测定仪结合离子导入仪检测微血管自律性舒缩活动的振幅。利用激光共聚焦显微镜、钙离子荧光探针Fluo-3AM检测ATP引起的RMMECs细胞内钙离子的变化及Rb1对其的影响。结果表明：试验确定了能够引起RMMECs细胞内游离钙离子释放的ATP的最低量为0.5 μg•mL^-1，瞬时升高差异显著的剂量是0.7 μg•mL^-1，能够影响RMMECs细胞内钙离子变化的GSRb1的最低量为8 μg•mL^-1。RMMECs经 8 μg•mL^-1 GSRb1孵育后，能够刺激细胞内游离钙离子释放的ATP的最低量升高为1.5 μg•mL^-1。本研究说明，提高微血管自律运动振幅的中药成分GSRb1能够通过对RMMECs胞内钙离子的影响，提高RMMECs对ATP的耐受能力（ATP的刺激量由0.5 μg•mL^-1升高到1.5 μg•mL^-1）。

Abstract:

The aim of this study was to investigate the effects of Ginsenoside Rb1(GSRb1) induce Hegu acupoint microvascular vasomotion and the effect on sensitive threshold of Rat myocardial microvascular endothelial cells(RMMECs).Microvascular vasomotion was detected by laser doppler blood flow monitor combined with iontophoresis device.The changes of calcium in the RMMECs were measured by laser scanning confocal microscopy combined with calcium fluorescent probe (Fluo-3AM).The results were as follows：The minimum amount of ATP could induce calcium transient increase in RMMECs was 0.5 μg•mL^-1，the dose of significant difference was 0.7 μg•mL^-1 and the minimum amounts of GSRb1 inducing calcium changes in RMMECs was 8 μg•mL^-1.After incubation 8 μg•mL^-1 of GSRb1，the minimum amount of ATP could induce calcium transient increase in RMMECs was 1.5 μg•mL^-1.Eight μg•mL^-1 of GSRb1 can inhibit the ATP-induced intracellular calcium transient rise，that meant，GSRb1 increased the sensitive threshold of RMMECs to ATP.

中图分类号:

王鑫，冯波，孙向婉，李平丽，董虹，穆祥. 人参皂苷Rb1对微血管自律性舒缩活动及大鼠心肌微血管内皮细胞内钙瞬时变化的影响[J]. 畜牧兽医学报, 2015, 46(1): 156-162.

WANG Xin，FENG Bo，SUN Xiang-wan，LI Ping-li，DONG Hong，MU Xiang. Effect of Ginsenoside Rb1 Induce Microvascular Vasomotion and the Effect on Sensitive Threshold of Rat Myocardial Microvascular Endothelial Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 156-162.

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参考文献

［1］修瑞娟.微血管自律运动的研究Ⅱ.骨骼肌的微血管自律运动及其与血流速度及血流量的关系(简报) ［J］.中国医学科学院学报,1985,7(2):116.
XIU R J.Study on microvascular vasomotion Ⅱ.Vasomotion in skeletal muscle and the relationship between it with blood flow and velocity of blood flow ［J］.Acta Academiae Medicinae Sinicae，1985，7(2):116.(in Chinese)
［2］SCHINDLER T H，NITZSCHE E U，OLSCHEWSKI M，et al.Chronic inflammation and impaired coronary vasoreactivity in patients with coronary risk factors ［J］.Circulation，2004，110(9):1069-1075.
［3］SIMMONS G H，PADILLA J，JENKINS N T，et al.Exercise training and vascular cell phenotype in a swine model of familial hypercholesterolaemia：conduit arteries and veins［J］.Exp Physiol，2014，99(2)：454-465.
［4］TIGNO X T,HANSEN B C,ALBANO A M.Vasomotion spectra and principal components of pooled measures predict diabetes in monkeys［J］.Int J Bifurcat Chaos，2009，19(7):2439-2446.
［5］HUNG M W，KRAVTSOV G M，LAU C F，et al.Melatonin ameliorates endothelial dysfunction，vascular inflammation，and systemic hypertension in rats with chronic intermittent hypoxia［J］.J Pineal Res，2013，55(3)：247-256.
［6］DE JONGH R T，SERNÉ E H，IJZERMAN R G，et al.Impaired local microvascular vasodilatory effects of insulin and reduced skin microvascular vasomotion in obese women［J］.Microvasc Res，2008，75(2)：256-262.
［7］宣颖超，张涛，郭洋，等.白头翁汤中主要成分导入合谷穴对皮肤微血管舒缩振幅的作用［J］.微循环学杂志，2011，21(2):1-3.
XUAN Y C，ZHANG T，GUO Y，et al.Effect of components of pulsatilla decoction on amplitude of cutaneous microvasomotion at Hegu Acupoint［J］.Chinese Journal of Microcirc Ulation，2011，21(2):1-3.(in Chinese)
［8］赵平平，洪俪凤.针灸对微血管自律运动(微循环)影响的研究概况［J］.甘肃中医，1999，12(2)：45-48.
ZHAO P P，HONG L F.Effect of acupuncture on microvascular vasomotion(microcirculation)［J］.Gansu Journal of Traditional Chinese Medicine，1999，12(2)：45-48.(in Chinese)
［9］赵雪，侯爽，黄群，等.半导体激光照射对穴位区微血管功能的影响［J］.激光杂志，2011，32(6)：64-66.
ZHAO X，HOU S，HUANG Q，et al.Effect of semiconductor laser radiation on point area microsvascular function［J］. Laser Journal，2011，32(6)：64-66.(in Chinese)
［10］ROSSI M，CARPI A，GALETTA F，et al.Skin vasomotion investigation：A useful tool for clinical evaluation of microvascular endothelial function?［J］.Biomed Pharmacother，2008，62(8):541-545.
［11］彭黎明，邓承祺.现代血栓与止血的实验室检测及其应用［M］.北京：人民卫生出版社，2004：277-284.
PENG L M，DENG C Q.Modern laboratory technology and its application in thrombosis and hemostasis［M］.Beijing:People’s Medical Publishing House,2004:277-284.(in Chinese)
［12］秦任甲.临床血液流变学(修订版)［M］.北京：北京大学医学出版社，2006.
QIN R J.Clinical hemorheology (Revision)［M］.Beijing:Peking University Medical Press,2006.(in Chinese)
［13］贾杰，周瑛，韦中奎.中药治疗心血管疾病研究概况［J］.北方药学，2014(2)：63-64.
JIA J，ZHOU Y，WEI Z K.The study of traditional Chinese medicine for cardiocerebrovascular disease［J］.Journal of North Pharmacy，2014(2)：63-64.(in Chinese)
［14］王梓宁.中医药治疗心血管病的文献计量分析［D］.北京：中国中医科学院，2013.
WANG Z N.The biblometric study on traditional Chinese medicine treatment of cardiovascular diseases［D］.Beijing:China Academy of Chinese Medicial Sciences,2013.(in Chinese)
［15］金戈，明海霞，董晓丽.益气活血中药对家兔心气虚证NO、ET及血液流变学的影响［J］.中国老年学杂志，2011，31(15)：2916-2918.
JIN G，MING H X，DONG X L.The effect of Chinese medicinal with supplementing qi and activating blood circulation in rabbit with syndrome of deficiency of qi in heart to NO,ET and hemorheology［J］.Chinese Journal of Gerontology，2011，31(15)：2916-2918.(in Chinese)
［16］HUANG T，LI Y，JIA S，et al.Capillary blood flow in patients with dysmenorrhea treated with acupuncture［J］. J Tradit Chin Med，2013，33(6)：757-760.
［17］曾昭炜，谢忠明，唐荣福，等.观察动物微循环的实验方法［J］.微循环学杂志，2011，21(3)：26-32.
ZENG Z W，XIE Z M，TANG R F，et al.The experimental technologies of obversing microcirculation in animals［J］.Chinese Journal of Microcirculation，2011，21(3)：26-32.(in Chinese)
［18］DESOUZA M D，BOUSKELA E.Arteriolar diameter and spontaneous vasomotion：Importance of potassium channels and nitric oxide［J］.Microvasc Res，2013，90：121-127.
［19］WANG H，ZHOU G，GAI H，et al.A fluorescein-based probe with high selectivity to cysteine over homocysteine and glutathione［J］.Chem Commun(Camb)，2012，48(67):8341-8343.
［20］MASHINO K，SADANAGA N，YAMAGUCHI H，et al.Expression of chemokine receptor CCR7 is associated with lymph node metastasis of gastric carcinoma ［J］.Cancer Res，2002，62(10)：2937-2941.
［21］KAHLENBERG J M，LUNDBERG K C，KERTESY S B，et al.Potentiation of caspase-1 activation by the P2X7 receptor is dependent on TLR signals and requires NF-kappaB-driven protein synthesis ［J］.J Immunol，2005，175(11)：7611-7622.
［22］张斌，金士翱，况铣.三七皂甙单体Rb1对心肌细胞膜钙离子通道的影响［J］.中国药理学通报，1998，14(1):33-35.
ZHANG B,JIN S A,KUANG X.Effect of Rb1 on calcium channel in guinea pig ventricular myocytes［J］.Chinese Pharmacological Bulletin,1998，14(1):33-35.(in Chinese)
［23］KIM S，NAH S Y，RHIM H.Neuroprotective effects of ginseng saponins against L- type Ca²⁺ channel-mediated cell death in rat cortical neurons［J］.Biochem Biophys Res Commun，2008，365(3):399-405.
［24］LOUGHREY C M，MACEACHERN K E，COOPER J，et al.Measurement of the dissociation constant of Fluo-3 for Ca²⁺ in isolated rabbit cardiomyocytes using Ca²⁺ wave characteristics［J］.Cell Calcium，2003，34(1):1-9.

人参皂苷Rb1对微血管自律性舒缩活动及大鼠心肌微血管内皮细胞内钙瞬时变化的影响

Effect of Ginsenoside Rb1 Induce Microvascular Vasomotion and the Effect on Sensitive Threshold of Rat Myocardial Microvascular Endothelial Cells

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