TPL2促进口蹄疫病毒在BHK-21细胞复制

doi:10.11843/j.issn.0366-6964.2018.06.012

畜牧兽医学报 ›› 2018, Vol. 49 ›› Issue (6): 1204-1211.doi: 10.11843/j.issn.0366-6964.2018.06.012

TPL2促进口蹄疫病毒在BHK-21细胞复制

魏南南, 徐守兴, 史喜娟, 卢炳州, 张克山*, 郑海学*, 刘湘涛

中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室国家口蹄疫参考实验室, 兰州 730046

收稿日期:2017-11-01 出版日期:2018-06-23 发布日期:2018-06-22
通讯作者: 张克山,男,副研究员,E-mail:zhangkeshan@caas.cn;郑海学,男,研究员,E-mail:zhenghaixue@caas.cn
作者简介:魏南南(1991-),女,河南焦作人,硕士生,主要从事FMDV致病机制研究
基金资助:
国家自然科学基金（NSFC-31572542）；国家科技支撑计划（2015BAD12B04）

TPL2 Enhances the Replication of Foot-and-mouth Disease Virus in BHK-21 Cells

WEI Nan-nan, XU Shou-xing, SHI Xi-juan, LU Bing-zhou, ZHANG Ke-shan*, ZHENG Hai-xue*, LIU Xiang-tao

State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

Received:2017-11-01 Online:2018-06-23 Published:2018-06-22

摘要/Abstract

摘要：

为研究肿瘤进行性位点2（TPL2）在仓鼠肾细胞（BHK-21）中对口蹄疫病毒（FMDV）复制的影响，使用FMDV感染BHK-21细胞，通过qRT-PCR技术检测FMDV感染对内源性TPL2转录水平的影响；根据GenBank公布的TPL2基因序列（NM007746.2）设计构建TPL2的真核表达质粒以及设计并合成宿主TPL2的RNAi序列，利用脂质体方法转染BHK-21细胞，通过qRT-PCR和Western blotting技术分析TPL2在BHK-21细胞过表达和干扰对FMDV复制的影响。结果表明，FMDV感染BHK-21细胞后，TPL2转录水平显著上调；过表达TPL2能够促进FMDV在BHK-21细胞中复制，而下调表达内源性TPL2后FMDV的复制受到抑制，表明TPL2促进FMDV在BHK-21细胞中进行复制，本结果进一步拓展了我们对FMDV与天然免疫机制之间关系的认识，同时为TPL2的相关研究积累了科学资料。

关键词: TPL2, 口蹄疫病毒, FMDV, BHK-21细胞, 天然免疫, 复制

Abstract:

This experiment was conducted to study the effects of tumor progression locus 2 (TPL2) on replication of foot-and-mouth disease virus (FMDV) in baby hamster kidney (BHK-21) cells. BHK-21 cells were infected by FMDV, the mRNA level of TPL2 was detected by qRT-PCR. TPL2 eukaryotic expression plasmid and RNAi sequences were designed and synthesized based on TPL2 sequences published in GenBank (NM007746.2). The effects of TPL2 on FMDV replication in BHK-21 cells were evaluated by qRT-PCR and Western blotting. The results indicated that the transcriptional level of TPL2 was significantly up-regulated during FMDV infection; Overexpression of TPL2 facilitated the replication of FMDV and downregulation of endogenous TPL2 inhibited FMDV replication. These results revealed that TPL2 promoted the proliferation of FMDV in BHK-21 cells. This research further expanded our understanding of the relationship between FMDV and the innate immune mechanism and had accumulated scientific data for the relevant research of TPL2.

Key words: TPL2, foot-and-mouth disease virus, FMDV, BHK-21 cell, innate immune, replication

中图分类号:

S852.659.6

魏南南, 徐守兴, 史喜娟, 卢炳州, 张克山, 郑海学, 刘湘涛. TPL2促进口蹄疫病毒在BHK-21细胞复制[J]. 畜牧兽医学报, 2018, 49(6): 1204-1211.

WEI Nan-nan, XU Shou-xing, SHI Xi-juan, LU Bing-zhou, ZHANG Ke-shan, ZHENG Hai-xue, LIU Xiang-tao. TPL2 Enhances the Replication of Foot-and-mouth Disease Virus in BHK-21 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(6): 1204-1211.

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TPL2促进口蹄疫病毒在BHK-21细胞复制

TPL2 Enhances the Replication of Foot-and-mouth Disease Virus in BHK-21 Cells

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