新疆野生荒漠肉苁蓉粗多糖对口蹄疫疫苗免疫小鼠的佐剂效应

doi:10.11843/j.issn.0366-6964.2017.08.019

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (8): 1535-1542.doi: 10.11843/j.issn.0366-6964.2017.08.019

新疆野生荒漠肉苁蓉粗多糖对口蹄疫疫苗免疫小鼠的佐剂效应

巴雪丽¹, 张爱莲^1*, 黄炯², 曹辉², 赵兵¹, 王丹阳¹

1. 新疆大学生命科学与技术学院, 新疆生物资源与基因工程重点实验室, 乌鲁木齐 830046;
2. 新疆天康畜牧生物技术股份有限公司, 乌鲁木齐 830000

收稿日期:2017-03-22 出版日期:2017-08-23 发布日期:2017-08-23
通讯作者: 张爱莲,女,副教授,E-mail:xjzal@163.com
作者简介:巴雪丽(1991-),女,河南周口人,硕士生,主要从事新型疫苗佐剂研究,E-mail:1909684104@qq.com
基金资助:
新疆维吾尔自治区科技援疆项目（201591150）

Adjuvant Effect of Xinjiang Wild Cistanche deserticola Y.C.Ma Crude Polysaccharides on Foot-and-mouth Disease Vaccines in Mice

BA Xue-li¹, ZHANG Ai-lian^1*, HUANG Jiong², CAO Hui², ZHAO Bing¹, WANG Dan-yang¹

1. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China;
2. Xinjiang Tiankang Livestock Biotechnology Co., Ltd, Urumqi 830000, China

Received:2017-03-22 Online:2017-08-23 Published:2017-08-23

摘要/Abstract

摘要：

探讨新疆野生荒漠肉苁蓉粗多糖（wild Cistanche deserticola Y.C.Ma crude polysaccharides，WCDCP）对口蹄疫（foot-and-mouth disease，FMD）疫苗免疫小鼠的佐剂效应及安全性。用不同剂量的WCDCP分别配伍FMDV灭活抗原及FMD疫苗，皮下注射ICR小鼠，免疫两次。ELISA法检测血清中IgG、IgG₁及IgG_2a抗体水平；流式细胞术检测淋巴结中CD4⁺CD44⁺和CD8⁺CD44⁺ T细胞表达情况。结果表明，中、高2个剂量的WCDCP均能显著增强FMDV灭活抗原特异性IgG的抗体水平（P<0.01）；中剂量的WCDCP显著增强了FMD疫苗特异性IgG及其IgG₁、IgG_2a抗体水平（P<0.05），而且具有长效性；WCDCP中剂量可以显著提高淋巴结中CD4⁺CD44⁺和CD8⁺CD44⁺ T细胞的表达水平（P<0.05）；WCDCP对小鼠的生长没有影响。总之，WCDCP可以长期增强FMD疫苗特异性抗体水平，显著增强T细胞免疫反应，对小鼠的生长无副作用。因此，WCDCP可以作为FMD疫苗的一种安全候选佐剂。

Abstract:

The aim of this study was to investigate the adjuvant effect and safety of wild Cistanche deserticola Y. C. Ma crude polysaccharides (WCDCP) on mice immunized with foot and mouth disease (FMD) vaccine. ICR mice were subcutaneously administrated twice with different doses of WCDCP co-administered with FMDV inactivated antigen or FMD vaccine, respectively. Antibody levels of IgG, IgG₁ and IgG_2a in serum were detected by ELISA. The expression levels of CD4⁺CD44⁺ and CD8⁺CD44⁺ T cells in lymph nodes were tested by flow cytometry. The result showed that medium and high dose of WCDCP could significantly enhance antibody level of FMDV-specific IgG in mice with FMDV inactivated antigen (P<0.01). Medium dose of WCDCP could enhance antibody level of FMDV-specific IgG, IgG₁ and IgG_2a in mice with FMDV vaccine (P<0.05), and long-term antibody level could be elicited. The expression levels of CD4⁺CD44⁺ and CD8⁺CD44⁺ T cells in lymph nodes was significantly higher than that in control group (P<0.05). WCDCP had no effect on the growth of mice. In conclusion, WCDCP can enhance FMDV-specific antibody levels over a long period, significantly enhance the T cell immune response and have no side effects. Therefore, WCDCP can be used as a safe candidate for FMD vaccines.

中图分类号:

S853.31

巴雪丽, 张爱莲, 黄炯, 曹辉, 赵兵, 王丹阳. 新疆野生荒漠肉苁蓉粗多糖对口蹄疫疫苗免疫小鼠的佐剂效应[J]. 畜牧兽医学报, 2017, 48(8): 1535-1542.

BA Xue-li, ZHANG Ai-lian, HUANG Jiong, CAO Hui, ZHAO Bing, WANG Dan-yang. Adjuvant Effect of Xinjiang Wild Cistanche deserticola Y.C.Ma Crude Polysaccharides on Foot-and-mouth Disease Vaccines in Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1535-1542.

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新疆野生荒漠肉苁蓉粗多糖对口蹄疫疫苗免疫小鼠的佐剂效应

Adjuvant Effect of Xinjiang Wild Cistanche deserticola Y.C.Ma Crude Polysaccharides on Foot-and-mouth Disease Vaccines in Mice

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