3种饲草不同配比的体外消化特性及组合效应分析

doi:10.11843/j.issn.0366-6964.2017.06.011

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (6): 1066-1075.doi: 10.11843/j.issn.0366-6964.2017.06.011

3种饲草不同配比的体外消化特性及组合效应分析

刘丽英¹, 王志军², 尹强³, 孙林⁴, 成启明¹, 范文强¹, 刘亚红⁴, 贾玉山^1*

1. 内蒙古农业大学草原与资源环境学院, 草地资源教育部重点实验室, 呼和浩特 010019;
2. 内蒙古自治区草原勘察规划院, 呼和浩特 010019;
3. 中国农业科学院草原研究所, 呼和浩特 010010;
4. 内蒙古自治区农牧业科学院, 呼和浩特 010031

收稿日期:2016-12-30 出版日期:2017-06-23 发布日期:2017-06-15
通讯作者: 贾玉山,教授,博士,博士生导师,E-mail:jys_nm@sina.com
作者简介:刘丽英(1983-),女,内蒙古呼和浩特人,博士生,主要从事牧草生产加工与利用研究,E-mail:365liying@163.com
基金资助:
内蒙古科技计划项目（20151113）；国家现代牧草产业技术体系项目（CARS-35）；内蒙古农牧科学院青年基金（2014QNJJM07）；“十三五”国家重点研发计划课题（2016YFC0500605）；“十二五”国家科技支撑计划课题（2012BAD13B07）

Research on the in vitro Digestibility and Associative Effects of Three Forage with Different Proportion

LIU Li-ying¹, WANG Zhi-jun², YIN Qiang³, SUN Lin⁴, CHENG Qi-ming¹, FAN Wen-qiang¹, LIU Ya-hong⁴, JIA Yu-shan^1*

1. Key Laboratory of Grassland Resource of Ministry of Education, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010019, China;
2. Inner Mongolia Autonomous Region Grassland and Planning Institute, Hohhot 010019, China;
3. Institute of Grassland Research of Chinese Academy of Agricultural Sciences, Hohhot 010010, China;
4. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China

Received:2016-12-30 Online:2017-06-23 Published:2017-06-15

摘要/Abstract

摘要：

本研究旨在实现低质粗饲料的有效利用，为我国北方地区饲草型发酵全混合日粮确定最佳粗饲料组合配方。将玉米秸、苜蓿、燕麦草3种饲草通过机械预处理，按不同比例混合后进行体外发酵，根据混合饲草的体外发酵指标（pH、IVDOM、GP、TVFA等）以及组合效应进行综合评定。结果表明，玉米秸+燕麦草组合体外发酵各指标及组合效应效果不佳；玉米秸+苜蓿干草组合中，处理组D（玉米秸∶苜蓿干草=60%∶40%）和处理组E（玉米秸∶苜蓿干草=50%∶50%）体外发酵指标（pH、IVDOM、GP、TVFA）值及正组合效应高于或显著高于其他组合（P<0.05）；玉米秸+苜蓿干草+燕麦草组合中，处理组R（玉米秸秆∶苜蓿干草∶燕麦干草=50%∶30%∶20%）和处理组Q（玉米秸秆∶苜蓿干草∶燕麦干草=50%∶40%∶10%）体外发酵指标值及正组合效应高于或显著高于其他组合（P<0.05）。综合考虑，玉米秸+苜蓿干草和玉米秸+苜蓿干草+燕麦草两个组合中，苜蓿的添加比例在30%以上时，体外发酵积累的pH、IVDOM、GP、TVFA值及组合效应显著增加，能明显地提高秸秆的消化率或降解率，饲草间产生正组合效应更显著。

关键词: 苜蓿干草, 混合饲草, 组合效应, 体外产气量, 体外发酵

Abstract:

The objective of this study was to effectively utilize roughage and determine the best roughage combination of fermented total mixed ration in northern China. The mixed forage was prepared with different proportions of corn stover, alfalfa hay and Avena sativa pretreated. The indexes of fermentation in vitro of mixed forage were determined by fermenting in vitro. The best combination of mixed forage was evaluated comprehensively by the indexes of fermentation in vitro and associative effects. The results showed that the in vitro fermentation effect of corn stover+Avena sativa combination was not good by analyzing the indexes of fermentation in vitro and associative effects. The indexes of fermentation in vitro (pH, IVDOM, GP and TVFA) and associative effects of the group D (corn stover:alfalfa hay =60%:40%) and group E (corn stover:alfalfa hay=50%:50%) were higher or significantly higher (P<0.05) than other groups in corn stover+alfalfa hay combination. The indexes of fermentation in vitro and associative effects of the group R (corn stover:alfalfa hay:Avena sativa=50%:30%:20%) and group Q (corn stover:alfalfa hay:Avena sativa=50%:40%:10%) were higher or significantly higher (P<0.05) than other groups in corn stover+alfalfa hay+Avena sativa combination. In conclusion, the indexes of fermentation in vitro and associative effects increased significantly when the proportion of adding alfalfa hay was more than 30% in corn stover+alfalfa hay and corn stover+alfalfa hay+Avena sativa combinations, which can significantly increased the digestibility and degradation rate of stover, the positive associative effects significantly increased.

Key words: alfalfa hay, mixed forage, associative effect, in vitro gas production, fermentation in vitro

中图分类号:

S816.52

刘丽英, 王志军, 尹强, 孙林, 成启明, 范文强, 刘亚红, 贾玉山. 3种饲草不同配比的体外消化特性及组合效应分析[J]. 畜牧兽医学报, 2017, 48(6): 1066-1075.

LIU Li-ying, WANG Zhi-jun, YIN Qiang, SUN Lin, CHENG Qi-ming, FAN Wen-qiang, LIU Ya-hong, JIA Yu-shan. Research on the in vitro Digestibility and Associative Effects of Three Forage with Different Proportion[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1066-1075.

参考文献

[1] 朱德文. 农作物秸秆用作动物饲料——可行性与限制因素分析[J]. 饲料研究, 2003(2):34-36.
ZHU D W. The feasibility and restriction factor analysis of crop straw used as animal feed[J]. Feed Research, 2003(2):34-36. (in Chinese)
[2] 冯伟, 黄力程, 李文才, 等. 我国农作物秸秆资源化利用的经济分析:一个理论框架[J]. 生态经济, 2011, 27(2):94-96, 115.
FENG W, HUANG L C, LI W C, et al. A theoretical framework for economic analysis of crops residues utilization in China[J]. Ecological Economy, 2011, 27(2):94-96, 115. (in Chinese)
[3] 梁榕旺, 徐淑莉. 我国秸秆资源现状及其利用[J]. 畜牧与饲料科学, 2011, 32(11):21-23.
LIANG R W, XV S L. Status and utilization of straw resource in China[J]. Animal Husbandry and Feed Science, 2011, 32(11):21-23. (in Chinese)
[4] 兰晓玲, 郭玉明, 贺俊林. 我国秸秆饲料化利用技术研究综述[J]. 安徽农学通报, 2013, 19(13):103-104.
LAN X L, GUO Y M, HE J L. Research review on the technology of stalk utilization in China[J]. Anhui Agricultural Science Bulletin, 2013, 19(13):103-104. (in Chinese)
[5] 刘庭玉. 饲草型全混日粮对肉羊生产性能影响研究[D]. 呼和浩特:内蒙古农业大学, 2012.
LIU T Y. Effects of performance of forage-type total mixed rations on mutton sheep[D]. Hohhot:Inner Mongolia Agricultural University, 2012. (in Chinese)
[6] 许红, 余莹, 梁新平, 等. 苜蓿干草对杜寒杂交羊瘤胃发酵参数及十二指肠食糜氨基酸含量的影响[J]. 动物营养学报, 2014, 26(6):1689-1697.
XU H, YU Y, LIANG X P, et al. Effects of dried alfalfa hay on ruminal fermentation parameters and amino acid contents in duodenal chyme of Dorper×Thin-tailed Han crossbred sheep[J]. Chinese Journal of Animal Nutrition, 2014, 26(6):1689-1697. (in Chinese)
[7] 张吉鹍, 李龙瑞, 吴文旋, 等. 稻草补饲苜蓿对山羊瘤胃发酵的组合效应[J]. 草业科学, 2014, 31(2):313-320.
ZHANG J K, LI L R, WU W X, et al. Associative effects of rice straw supplemented with different level of alfalfa hay on rumen fermentation of goats[J]. Pratacultural Science, 2014, 31(2):313-320. (in Chinese)
[8] 张亚格, 李茂, 周汉林, 等. 豆科牧草在肉羊生产中的应用[J]. 家畜生态学报, 2016, 37(5):73-77.
ZHANG Y G, LI M, ZHOU H L, et al. Application of leguminous forages in goat production[J]. Acta Ecologiae Animalis Domastici, 2016, 37(5):73-77. (in Chinese)
[9] 李纪委, 赵俊, 赵本领, 等. TMR饲喂技术在肉羊养殖中的应用[J]. 安徽农业科学, 2016, 44(9):124-125, 179.
LI J W, ZHAO J, ZHAO B L, et al. Application of total mixed ration feeding technology in mutton sheep breeding[J]. Journal of Anhui Agricultural Sciences, 2016, 44(9):124-125, 179. (in Chinese)
[10] 薛红枫, 任丽萍, 孟庆翔. 不同玉米秸秆类型碳水化合物组分体外发酵动态分析[J]. 畜牧兽医学报, 2010, 41(9):1117-1125.
XUE H F, REN L P, MENG Q X. In vitro fermentation kinetic analysis of the carbohydrate fractions in various types of corn stalks[J]. Acta Veterinaria et Zootechnica Sinica, 2010, 41(9):1117-1125. (in Chinese)
[11] 闫贵龙, 孟庆翔, 陈绍江. 高油298玉米秸秆的适宜收获期及其营养价值与其他品种玉米秸秆的比较优势[J]. 畜牧兽医学报, 2006, 37(3):250-256.
YAN G L, MENG Q X, CHEN S J. Optimum harvest time of high oil corn 298 stalks and their superiority in nutritional value comparing with other corn variety stalks[J]. Acta Veterinaria et Zootechnica Sinica, 2006, 37(3):250-256. (in Chinese)
[12] 丁雪, 张幸怡, 郝小燕, 等. 玉米秸秆不同部位的蛋白质营养价值与其分子结构相关关系的研究[J]. 畜牧兽医学报, 2016, 47(8):1592-1600.
DING X, ZHANG X Y, HAO X Y, et al. Protein molecular structure in relation to protein nutritive values at different sections of Corn Stover[J]. Acta Veterinaria et Zootechnica Sinica, 2016, 47(8):1592-1600. (in Chinese)
[13] 郭旭生. 秸秆生化复合处理技术及系统营养调控在肉羊日粮配制中的应用研究[D]. 银川:宁夏大学, 2004.
GUO X S. Study on biochemical treatment technique for crop foddor and application of systematic nutrient manipulation in formulating total mixed rations of mutton sheep[D]. Yinchuan:Ningxia University, 2004. (in Chinese)
[14] 王晓光. 饲草型全混日粮饲用价值评价研究[D]. 呼和浩特:内蒙古农业大学, 2011.
WANG X G. Study on feeding evaluation of forage-type total mixed rations[D]. Hohhot:Inner Mongolia Agricultural University, 2011. (in Chinese)
[15] 王璐璐. 秸秆饲粮化的初步研究[D]. 无锡:江南大学, 2014.
WANG L L. Preliminary study on preparation of feedstuffs from straw[D]. Wuxi:Jiangnan University, 2014. (in Chinese)
[16] 成立新, 贾玉山, 杨瑞杰, 等. 饲草型全混日粮评价与组合效应研究[J]. 饲料研究, 2013(5):42-46.
CHENG L X, JIA Y S, YANG R J, et al. The effect of evaluation and combination research on forage type total mixed ration[J]. Feed Research, 2013(5):42-46. (in Chinese)
[17] 李成会, 李建国, 单文辉, 等. 裹包青贮技术在产奶牛用全混合日粮贮存中的应用研究[J]. 中国奶牛, 2011(14):15-18.
LI C H, LI J G, SHAN W H, et al. Study of baling storage technology in storage of milk cow's total mixed ration[J]. China Dairy Cattle, 2011(14):15-18. (in Chinese)
[18] MENKE K H, STEINGASS H. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J]. Anim Res Dev, 1988, 28(1):7-55.
[19] 荣磊. 苜蓿草捆贮藏品质调控方法研究[D]. 呼和浩特:内蒙古农业大学, 2013.
RONG L. Study on the control methods of storage quality for alfalfa bale[D]. Hohhot:Inner Mongolia Agricultural University, 2013. (in Chinese)
[20] MENKE K H, RAAB L, SALEWSK A, et al. The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro[J]. J Agric Sci, 1979, 93(1):217-222.
[21] NSAHLAI I V, UMUNNA N N, NEGASSA D. The effect of multi-purpose tree digesta on in vitro gas production from napier grass or neutral-detergent fibre[J]. J Sci Food Agric, 1995, 69(4):519-528.
[22] MAURICIO R M, MOULD F L, DHANOA M S, et al. A semi-automated in vitro gas production technique for ruminant feedstuff evaluation[J]. Anim Feed Sci Technol, 1999, 79(4):321-330.
[23] 王旭. 利用GI技术对粗饲料进行科学搭配及绵羊日粮配方系统优化技术的研究[D]. 呼和浩特:内蒙古农业大学, 2003.
WANG X. A technique for formulation of mixed forages by grading index and systematic optimization of sheep ration based on the technique[D]. Hohhot:Inner Mongolia Agricultural University, 2003. (in Chinese)
[24] 赵旭昌, 王哲, 赵建军, 等. 健康牛、羊瘤胃内环境参数[J]. 中国兽医学报, 1997, 17(5):483-485.
ZHAO X C, WANG Z, ZHAO J J, et al. Parameters of internal environment of rumen in cattle and sheep[J]. Chinese Journal of Veterinary Science, 1997, 17(5):483-485. (in Chinese)
[25] 王桂瑛, 毛华明, 文际坤. 玉米的不同加工处理对瘤胃液VFA浓度的影响[J]. 饲料工业, 2008, 29(1):44-46.
WANG G Y, MAO H M, WEN J K. Effect of different corn processing on the VFA concentration in rumen liquid of ruminants[J]. Feed Industry, 2008, 29(1):44-46. (in Chinese)
[26] OBITSU T, GOTO M, SUGINO T, et al. The effect of dietary ratios of corn silage and alfalfa hay on carbohydrate digestion and retention time of feed particles in the gastrointestinal tract of steers[J]. J Anim Sci, 2009, 80(5):546-555.
[27] DORAN M P, LACA E A, SAINZ R D. Total tract and rumen digestibility of mulberry foliage (Morus alba), alfalfa hay and oat hay in sheep[J]. Anim Feed Sci, 2007, 138(3-4):239-253.
[28] LIU J X, SUSENBETH A, SüDEKUM K H. In vitro gas production measurements to evaluate interactions between untreated and chemically treated rice straws, grass hay, and mulberry leaves[J]. J Anim Sci, 2002, 80(2):517-524.
[29] 谭支良, 卢德勋. 提高粗饲料利用效率的系统组合营养技术及其组合效应的研究进展[J]. 饲料博览, 1999, 11(7):6-10.
TAN Z L, LU D X. The research progress in system combination nutrition technology to improve the utilization efficiency of roughage and combined effect[J]. Feed Review, 1999, 11(7):6-10. (in Chinese)
[30] 周传社, 汤少勋, 姜海林, 等. 农作物秸秆体外发酵营养特性及其组合利用研究[J]. 应用生态学报, 2005, 16(10):1862-1867.
ZHOU C S, TANG S X, JIANG H L, et al. In vitro fermentation characteristics of crop straws and their combined utilization[J]. Chinese Journal of Applied Ecology, 2005, 16(10):1862-1867. (in Chinese)
[31] 周卫东, 黄新, 王亚琴, 等. 不同处理方法对自然晒制苜蓿干草的影响[J]. 草业科学, 2006, 23(2):43-46.
ZHOU W D, HUANG X, WANG Y Q, et al. Effect of different treatments on natural drying alfalfa[J]. Pratacultural Science, 2006, 23(2):43-46. (in Chinese)

3种饲草不同配比的体外消化特性及组合效应分析

Research on the in vitro Digestibility and Associative Effects of Three Forage with Different Proportion

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