猪圆环病毒3型检测及其Cap结构序列和抗原性预测分析

doi:10.11843/j.issn.0366-6964.2017.06.012

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (6): 1076-1084.doi: 10.11843/j.issn.0366-6964.2017.06.012

猪圆环病毒3型检测及其Cap结构序列和抗原性预测分析

湛洋^1,2#, 王东亮^1,2#, 王乃东^1,2, 蒋一凡^1,2, 黄坤^1,2, 崔尚金³, 姜平^4,5, 杨毅^1,2,5*

1. 湖南农业大学动物医学院, 长沙 410128;
2. 湖南农业大学逆向动物疫苗研究中心及功能蛋白质组学实验室, 长沙 410128;
3. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
4. 南京农业大学动物医学院, 南京 210095;
5. 江苏南农高科技股份有限公司, 江阴 214405

收稿日期:2017-01-09 出版日期:2017-06-23 发布日期:2017-06-15
通讯作者: 杨毅,教授,E-mail:yiyang@hunau.edu.cn
作者简介:湛洋(1989-),男,湖南长沙人,博士,主要从事兽医功能蛋白质的研究,E-mail:yangzhan@hunau.edu.cn,Tel:0731-84635276;王东亮(1993-),男,湖南永州人,硕士,E-mail:dongliangwang2015@163.com,Tel:0731-84635276
基金资助:
湖南省教育厅重点项目（15A086）；湖南省研究生科研创新项目（CX2016B285；CX2016B314）

Survey on Detection and Analyses of Cap Antigenicity Prediction of Porcine Circovirus Type 3 Isolated from Partial Provinces of Southern China

ZHAN Yang^1,2, WANG Dong-liang^1,2#, WANG Nai-dong^1,2#, JIANG Yi-fan^1,2, HUANG Kun^1,2, CUI Shang-jin³, JIANG Ping^4,5, YANG Yi^1,2,5*

1. College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China;
2. Research Center of Reverse Vaccinology and Laboratory of Functional Proteomics, Changsha 410128, China;
3. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
4. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;
5. Jiangsu Nannong Hi-tech Co., LTD., Jiangyin 214405, China

Received:2017-01-09 Online:2017-06-23 Published:2017-06-15

摘要/Abstract

摘要：

近期报道在美国多个州的养猪场发现了一种新型的猪圆环病毒3型毒株（porcine circovirus 3，PCV3）。为了确定PCV3是否已经在我国南方部分省份的猪场中广泛存在，并对目前PCV2疫苗是否对PCV3感染具有交叉保护作用作出科学预测，笔者对我国南方几个省份疑似猪皮炎肾病综合征的病猪组织样品进行PCV3巢式PCR检测及其核衣壳蛋白质（capsid protein，Cap）结构的PyMol和抗原表位服务器在线模拟分析。结果首次在这些病料中检测到PCV3的存在，部分病料表现为PCV2和PCV3共感染的特性。对其Cap结构及抗原性分析发现，PCV2和PCV3表现出很大的差异。因此，推测PCV2和PCV3不具有交叉免疫保护的特性，新的疫苗研究和开发，对于将来控制PCV3流行是必要的。

关键词: 猪圆环病毒3型, 皮炎肾病综合征, 巢式PCR, 核衣壳蛋白质

Abstract:

A novel genotype of porcine circovirus type 3 (PCV3) has recently been found to be widely distributed in several states in America. In order to confirm that whether PCV3 has been distributed in southern China, and make prediction about whether the PCV2 vaccines have protective effect on PCV3. In this study, nested-PCR was used for detection of PCV3 samples collected from partial provinces of southern China, PyMol and web server were used to analyze the structure of the PCV3 capsid protein (Cap). PCV3 was detected in the pigs with PDNS-like syndrome, and these pigs in farms of several provinces of southern China were co-infected with both PCV 2 and PCV 3. Analyses of the primary sequences and the 3-Dimensional (3D) structures of both PCV 2 and PCV 3 Cap demonstrates large, distinct differences in their antigenic epitopes, suggesting cross-protection between PCV2 and PCV3 is impossible. Therefore, the development of novel vaccines will most likely be required to control PCV3 prevalence and infections on farms.

Key words: porcine circovirus type 3 (PCV3), porcine dermatitis and nephropathy syndrome (PDNS), nested-PCR, capsid protein

中图分类号:

S852.659.2

湛洋, 王东亮, 王乃东, 蒋一凡, 黄坤, 崔尚金, 姜平, 杨毅. 猪圆环病毒3型检测及其Cap结构序列和抗原性预测分析[J]. 畜牧兽医学报, 2017, 48(6): 1076-1084.

ZHAN Yang, WANG Dong-liang, WANG Nai-dong, JIANG Yi-fan, HUANG Kun, CUI Shang-jin, JIANG Ping, YANG Yi. Survey on Detection and Analyses of Cap Antigenicity Prediction of Porcine Circovirus Type 3 Isolated from Partial Provinces of Southern China[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(6): 1076-1084.

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猪圆环病毒3型检测及其Cap结构序列和抗原性预测分析

Survey on Detection and Analyses of Cap Antigenicity Prediction of Porcine Circovirus Type 3 Isolated from Partial Provinces of Southern China

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