脂多糖对体外培养猪颗粒细胞增殖、凋亡和雌二醇分泌的影响

doi:10.11843/j.issn.0366-6964.2016.04.008

摘要/Abstract

摘要：

旨在探讨脂多糖（LPS）对猪颗粒细胞增殖、凋亡及雌二醇（E₂）分泌的影响。采用不同浓度的LPS（0、500、1 000和2 000 ng•mL^-1）处理体外培养的猪颗粒细胞，并测定细胞增殖、凋亡及相关基因FN1、IGF2、IGFBP2、Cyclin D1、Cyclin D2和P27^kip的表达，同时对E₂的分泌及P450arom的表达进行检测。结果表明，1 000或2 000 ng•mL^-1 LPS均可显著促进颗粒细胞增殖、抑制细胞凋亡、提高活细胞百分率（P<0.05）。而500 ng•mL^-1 LPS即可促进与细胞生长增殖、周期相关基因FN1（P<0.01），IGF2、IGFBP2（P<0.05），Cyclin D1、Cyclin D2（P<0.01）的表达，抑制阻碍细胞周期的基因P27^kip（P<0.01）的表达，同时可以通过强烈抑制芳香化酶P450arom基因的表达（P<0.01），显著抑制E₂的分泌（P<0.01）。综上表明，LPS可以促进颗粒细胞增殖并抑制细胞凋亡，同时抑制颗粒细胞分泌E₂的功能。

关键词: 颗粒细胞, LPS, 细胞增殖, 细胞凋亡, 基因表达, E₂

Abstract:

In order to study the effects of Lipopolysaccharide（LPS） on proliferation，apoptosis and estradiol (E₂) secretion of porcine granulosa cells.Primary porcine granulosa cells were cultured in vitro and treated by LPS at different concentrations (0，500，1 000 and 2 000 ng•mL^-1).Then cell proliferation，apoptosis，secretion of E₂ and related gene expressions，FN1，IGF2，IGFBP2，Cyclin D1，Cyclin D2，P27^kip and P450arom were determined individually.The results showed that cell proliferation and survival ratio were significantly promoted at LPS concentrations of 1 000 or 2 000 ng•mL^-1(P<0.05)，on the contrary，cell apoptosis was inhibited.However，gene expression levels of FN1 (P<0.01)，IGF2，IGFBP2 (P<0.05)，Cyclin D1 and Cyclin D2 (P<0.01) were up-regulated at 500 ng•mL^-1 of LPS，P27^kip (P<0.01) which hindered cell cycle was inhibited.P450arom gene expression was decreased，resulting the inhibition of E₂secretion when treated by LPS.These results suggested that granulosa cell proliferation could be promoted by LPS，while cell apoptosis and E₂secretion were inhibited.

Key words: granulosa cells, LPS, cell proliferation, cell apoptosis, gene expression, E₂

中图分类号:

S828
S814.1

果双双，李辉，施振旦，马卫明. 脂多糖对体外培养猪颗粒细胞增殖、凋亡和雌二醇分泌的影响[J]. 畜牧兽医学报, 2016, 47(4): 693-699.

GUO Shuang-shuang，LI Hui，SHI Zhen-dan，MA Wei-ming. Effects of Lipopolysaccharide on Proliferation，Apoptosis and Estradiol Secretion of in vitro Cultured Porcine Granulosa Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 693-699.

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