野生水禽源ClassⅠ新城疫病毒对SPF鸡的致病性研究

doi:10.11843/j.issn.0366-6964.2015.09.016

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (9): 1606-1612.doi: 10.11843/j.issn.0366-6964.2015.09.016

野生水禽源ClassⅠ新城疫病毒对SPF鸡的致病性研究

杨少华，许传田，黄艳艳，张琳，黄庆华，胡北侠，朱曼玲，张秀美^*

（山东省农业科学院畜牧兽医研究所山东省畜禽疫病防治与繁育重点实验室，济南 250100）

收稿日期:2014-12-06 出版日期:2015-09-23 发布日期:2015-09-22
通讯作者: 张秀美（1956-），女，江苏徐州人，研究员，主要从事禽病综合防控技术研究，E-mail：zxm820410@163.com
作者简介:杨少华（1978-），女，山东烟台人，硕士，助理研究员，主要从事禽病综合防控技术研究，Tel:0531-88622611，E-mail：ysh7865@163.com
基金资助:
公益性行业（农业）科研专项（201003012；201303033）；现代农业产业技术体系建设专项资金（CARS-42-Z12）

Research on Pathogenicity of SPF Chicken Infected with a Class Ⅰ NDV Isolated from Waterfowl

YANG Shao-hua，XU Chuan-tian，HUANG Yan-yan，ZHANG Lin，HUANG Qing-hua，HU Bei-xia，ZHU Man-ling，ZHANG Xiu-mei^*

(Shandong Key Lab of Animal Disease Control and Breeding，Institute of Animal Science and Veterinary Medicine，Shandong Academy of Agricultural Sciences，Jinan 250100,China)

Received:2014-12-06 Online:2015-09-23 Published:2015-09-22

摘要/Abstract

摘要：

为了科学地了解新城疫病毒（NDV）的遗传进化规律，本研究对从鹭科候鸟分离的NDV毒株 SD22/13的生物学特性和致病特性进行了研究。通过致病性指数测定和交叉血凝抑制试验测定分离株SD22/13的毒力及与LaSota疫苗株、基因Ⅶ型NDV毒株的抗原相关性，同时设计引物对F基因进行克隆测序和遗传进化分析，并对其在SPF鸡上的致病性进行评价。生物学特性和遗传进化分析显示SD22/13属于ClassⅠ分支基因3型，与LaSota疫苗株的抗原相关性为56.1%，与基因Ⅶ型强毒株的抗原相关性低于50%。致病性试验结果表明该毒株可在鸡体内较好复制，并刺激机体产生较高水平血清抗体，鸡的免疫器官和肾虽均有轻微的病理损伤，但无发病死亡，对试验鸡致病性较低；SD22/13感染后30 d，再以基因Ⅶ型强毒株攻毒，在10 d观察期内SD22/13感染组鸡均健康，无发病死亡，而对照组鸡至第5天时全部死亡。本研究结果表明分离株SD22/13在生物学特性、抗原性和对SPF鸡致病性方面与基因Ⅶ型NDV存在明显差异；虽然SD22/13与基因Ⅶ强毒株抗原相关性较低，不能阻止其排毒，却能完全保护鸡抵抗强毒株的攻击。

关键词: 新城疫病毒, 野生水禽, 致病性

Abstract:

A lentogenic Newcastle disease virus (NDV)，SD22/13，was isolated from heron.The biology and pathogenicity were characterized in order to understand the evolution of NDV better.F gene of SD22/13 was sequenced and compared with that of reference strains.The antigenicity of the virus was compared with that of LaSota and other genotype Ⅶ virulent NDV strains via cross hemagglutination inhibition (HI).The pathogenicity of SD22/13 to SPF chicken was also conducted.The results indicated that SD22/13 was classified as a class Ⅰ genotype 3 NDV based on a partial sequence of the F gene.The antigenicity correlation between SD22/13 and LaSota was 56.1%，and the correlation of which with genotype Ⅶ NDV strains was less than 50%.Experiments on animals demonstrate that the isolate SD22/13 can replicate well in SPF chicken and induce a high immune antibody and had slight pathogenic to immune organ and kidney.Chickens inoculated with SD22/13 were challenged with genotype Ⅶ virulence NDV strain on 30dpi，and there had no death in chickens infected with SD22/13.The results demonstrated that SD22/13 stain had significant differences with genotypeⅦ NDV in biological characterization and pathogenic to chickens.Although the antigenic correlation was low between SD22/13 and genotype Ⅶ NDV，class I viruses can provide 100% protection rate，but it cannot block virus shedding upon challenge with genotypeⅦ virulent NDV.

Key words: Newcastle disease virus, wild waterfowl, pathogenicity

中图分类号:

S852.659.5

杨少华，许传田，黄艳艳，张琳，黄庆华，胡北侠，朱曼玲，张秀美. 野生水禽源ClassⅠ新城疫病毒对SPF鸡的致病性研究[J]. 畜牧兽医学报, 2015, 46(9): 1606-1612.

YANG Shao-hua，XU Chuan-tian，HUANG Yan-yan，ZHANG Lin，HUANG Qing-hua，HU Bei-xia，ZHU Man-ling，ZHANG Xiu-mei. Research on Pathogenicity of SPF Chicken Infected with a Class Ⅰ NDV Isolated from Waterfowl[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1606-1612.

参考文献

［1］ALEXANDER D J，SAIF J M，BARNES H J，et al.Newcastle disease，other avian paramyxoviruses and pneumovirus infections［M］// SAIF Y M，BARNES H J，GLISSON J R，et al.Disease of Poultry.11 edition.Ames，IA：Iowa State University Press，2003:63-99.
［2］MAYO M A.Virus taxonomy-Houston 2002［J］. Arch Virol，2002，147(5):1071-1076.
［3］KATTENBELT J A，STEVENS M P，GOUD A R.Sequence variation in the Newcastle disease virus genome［J］.Virus Res，2006,116(1-2):168-184.
［4］HUANG Y，WAN H Q，LIU H Q，et al.Genomic sequence of an isolate of Newcastle disease virus isolated from an outbreak in geese：a novel six nucleotide insertion in the non-coding region of the nucleoprotein gene［J］. Arch Virol，2004,149(7):1445-1457.
［5］ZOU J，SHAN S,YAO N，et al.Complete genome sequence and biological characterizations of a novel goose paramyxovirus-SF02 isolated in China［J］.Virus Genes，2005，30(1):13-21.
［6］KIM L M，KING D J，CURRY P E，et al.Phylogenetic diversity among low-virulence newcastle disease viruses from waterfowl and shorebirds and comparison of genotype distributions to those of poultry-origin isolates［J］.J Virol，2007，81(22):12641-12653.
［7］TAKAKUWA H，ITO T，TAKADA A，et al.Potentially virulent Newcastle disease viruses are maintained in migratory waterfowl populations［J］.Jpn J Vet Res，1998，45(4)：207-215.
［8］KIM L M，KING D J，SUAREZ D L，et al.Characterization of class I Newcastle disease virus isolates from Hong Kong live bird markets and detection using real-time reverse transcription-PCR［J］.J Clin Microbiol，2007，45(4)：1310-1314.
［9］杨少华,胡北侠,许传田，等.三株新城疫病毒强毒株的生物学特性及全基因组序列分析［J］.病毒学报，2012,28(2):143-150.
YANG S H，HU B X，XU C T，et al.Biological characteristics of three Newcastle disease virus isolates and entire genome sequences analysis［J］.Chinese Journal of Virology，2012，28(2):143-150.（in Chinese）
［10］YIN R，DING Z，LIU X，et al.Inhibition of Newcastle disease virus replication by RNA interference targeting the matrix protein gene in chicken embryo fibroblasts［J］.J Virol Methods，2010，167(1):107-111.
［11］LIU X，WANG X，WU S，et al.Surveillance for avirulent Newcastle disease viruses in domestic ducks (Anas platyrhynchos and Cairina moschata) at live bird markets in Eastern China and characterization of the viruses isolated［J］.Avian Pathol，2009，38(5)：377-391.
［12］ALEXANDER D J，CAMPBELL G，MANVELL R J，et al．Characterisation of an antigenically unusual virus responsible for two outbreaks of Newcastle disease in the Republic of Ireland in 1990［J］.Vet Rec，1992，130(4)：65-68．
［13］HU B，HUANG Y，HE Y，et al.Avian influenza virus and Newcastle disease virus (NDV) surveillance in commercial breeding farm in China and the characterization of Class I NDV isolates［J］.Vet Microbiol，2010，144(1-2):82-86.
［14］赵晨辰.中国农业科学院中国部分地区2010～2012年新城疫病毒分子流行病学研究［D］.哈尔滨:中国农业科学院，2013.
ZHAO C C.Molecular epidemiology of Newcastle disease virus isolated in partial region of China during 2010-2012［D］.Harbin：Chinese Academy of Agriculture Science，2013.(in Chinese)
［15］MENG C C，QIU X，JIN S，et al.Whole genome sequencing and biological characterization of Duck/JS/10，a new lentogenic class I Newcastle disease virus［J］.Arch Virol，2012，157(5):869-880.
［16］SHENGQING Y，KISHIDA N，ITO H，et al.Generation of velogenic Newcastle disease viruses from a nonpathogenic waterfowl isolate by passaging in chickens［J］.Virology，2002，301(2):206-211.

[1]	武炜, 王飒, 孟春春, 仇旭升, 廖瑛, 谭磊, 宋翠萍, 刘炜玮, 孙英杰, 丁铲. 应用CRISPR/Cas9敲除HeLa细胞DDX21基因对新城疫病毒复制的影响[J]. 畜牧兽医学报, 2019, 50(7): 1433-1440.
[2]	武炜, 王飒, 孟春春, 仇旭升, 廖瑛, 谭磊, 宋翠萍, 刘炜玮, 孙英杰, 丁铲. 应用CRISPR/Cas9敲除HeLa细胞DDX21基因对新城疫病毒复制的影响[J]. 畜牧兽医学报, 2019, 50(7): 1433-1440.
[3]	于可响, 刘存霞, 宫晓, 路晓, 胡峰, 郭效珍, 马秀丽, 李玉峰, 黄兵, 宋敏训. 一株肉鸡呼肠孤病毒变异株的分离鉴定[J]. 畜牧兽医学报, 2019, 50(5): 1039-1047.
[4]	于可响, 刘存霞, 宫晓, 路晓, 胡峰, 郭效珍, 马秀丽, 李玉峰, 黄兵, 宋敏训. 一株肉鸡呼肠孤病毒变异株的分离鉴定[J]. 畜牧兽医学报, 2019, 50(5): 1039-1047.
[5]	许程志, 吴运谱, 贾云慧, 杨时鳗, 何利昆, 陈艳, 周晨阳, 杨焕良, 乔传玲, 陈化兰. 一株重配型H1N2猪流感病毒的进化分析及对小鼠的致病性[J]. 畜牧兽医学报, 2019, 50(4): 802-810.
[6]	邓志文, 王泽平, 李倩文, 尹磊, 涂健, 宋祥军, 祁克宗. 禽致病性大肠杆菌双组分系统phoP/Q对鞭毛Ⅲ型分泌系统基因的调控预测[J]. 畜牧兽医学报, 2019, 50(4): 821-829.
[7]	许程志, 吴运谱, 贾云慧, 杨时鳗, 何利昆, 陈艳, 周晨阳, 杨焕良, 乔传玲, 陈化兰. 一株重配型H1N2猪流感病毒的进化分析及对小鼠的致病性[J]. 畜牧兽医学报, 2019, 50(4): 802-810.
[8]	邓志文, 王泽平, 李倩文, 尹磊, 涂健, 宋祥军, 祁克宗. 禽致病性大肠杆菌双组分系统phoP/Q对鞭毛Ⅲ型分泌系统基因的调控预测[J]. 畜牧兽医学报, 2019, 50(4): 821-829.
[9]	胡焱, 段志强, 嵇辛勤, 赵佳福, 邓珊珊, 李世静, 熊建民. GST pull-down验证新城疫病毒基质蛋白与鸡importin β1蛋白的相互作用[J]. 畜牧兽医学报, 2019, 50(1): 126-133.
[10]	杨斯琴, 敖日格乐, 王纯洁, 陈玉洁. 阿如拉-7味散对感染致病性E.coli O₁大鼠肠黏膜屏障的影响[J]. 畜牧兽医学报, 2019, 50(1): 193-202.
[11]	胡焱, 段志强, 嵇辛勤, 赵佳福, 邓珊珊, 李世静, 熊建民. GST pull-down验证新城疫病毒基质蛋白与鸡importin β1蛋白的相互作用[J]. 畜牧兽医学报, 2019, 50(1): 126-133.
[12]	杨斯琴, 敖日格乐, 王纯洁, 陈玉洁. 阿如拉-7味散对感染致病性E.coli O₁大鼠肠黏膜屏障的影响[J]. 畜牧兽医学报, 2019, 50(1): 193-202.
[13]	高照, 胡娇, 刘秀梵. 两株小鼠致病性差异显著的H5N1禽流感病毒PA蛋白差异互作宿主蛋白研究[J]. 畜牧兽医学报, 2018, 49(9): 1952-1960.
[14]	贺美玲, 王纯洁, 贾知锋, 斯木吉德, 敖日格乐. 高通量测序分析Nisin对腹泻小鼠肠道菌群的影响[J]. 畜牧兽医学报, 2018, 49(9): 2015-2024.
[15]	高照, 胡娇, 刘秀梵. 两株小鼠致病性差异显著的H5N1禽流感病毒PA蛋白差异互作宿主蛋白研究[J]. 畜牧兽医学报, 2018, 49(9): 1952-1960.

野生水禽源ClassⅠ新城疫病毒对SPF鸡的致病性研究

Research on Pathogenicity of SPF Chicken Infected with a Class Ⅰ NDV Isolated from Waterfowl

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价