RNAi对羊驼黑色素细胞中MC1R表达量及黑色素合成的影响

doi:10.11843/j.issn.0366-6964.2015.07.012

畜牧兽医学报 ›› 2015, Vol. 46 ›› Issue (7): 1163-1168.doi: 10.11843/j.issn.0366-6964.2015.07.012

RNAi对羊驼黑色素细胞中MC1R表达量及黑色素合成的影响

孙笑尉，张雪莲，庞全海^*，李振，赵礼军，陈娟娟，牛峰

（山西农业大学动物科技学院，太谷 030801）

收稿日期:2014-12-19 出版日期:2015-07-23 发布日期:2015-07-20
通讯作者: 庞全海，教授，E-mail：pangquanhai@126.com
作者简介:孙笑尉（1990-），女，山东莱西人，硕士生，主要从事动物干细胞生物学研究，E-mail:sxw9007@163.com
基金资助:
国家自然科学基金（30972223；31272628）

Effect of RNAi on MC1R Expression and Melanin Synthesis in the Melanocytes of Alpaca

SUN Xiao-wei，ZHANG Xue-lian，PANG Quan-hai^*，LI Zhen，ZHAO Li-jun，CHEN Juan-juan，NIU Feng

(College of Animal Science and Veterinary Medicine，Shanxi Agricultural University，Taigu 030801，China)

Received:2014-12-19 Online:2015-07-23 Published:2015-07-20

摘要/Abstract

摘要：

旨在研究RNAi 对羊驼黑色素细胞中MC1R 表达量及黑色素合成的影响。合成3对MC1R siRNA，将MC1R siRNA瞬时转染羊驼黑色素细胞，qRT-PCR法检测MC1R mRNA表达量，细胞免疫组化技术对MC1R进行定位，利用吸光度值检测黑色素细胞中黑色素的合成量。结果显示，siRNA2 组和siRNA3 组的MC1R mRNA 相对表达量极显著低于空白对照组（P<0.01），siRNA1组和阴性对照组与空白对照组相比差异不显著（P>0.05），siRNA2、siRNA3 两组的黑色素合成量低于空白对照组，差异极显著（P<0.01）。将MC1R siRNA 成功转染到羊驼黑色素细胞中，筛选出有效MC1R siRNA，MC1R的抑制情况与黑色素合成量一致，说明黑色素细胞中MC1R 对黑色素的合成具有调控作用，其作用机制需进一步研究。

关键词: 小干扰RNA, 黑色素皮质素受体1基因, 羊驼, 黑色素细胞, 黑色素

Abstract:

The objectives of this study were to evaluate the effects of RNAi on MC1R expression and melanin synthesis in the melanocytes of alpaca.Three pairs of specific siRNA for MC1R gene were designed for RNA interference in the melanocytes of alpaca.The expression of MC1R siRNA was detected by using qRT-PCR technology.The location of MC1R was detected by immunohistochemistry.The amount of melanin synthesis in melanocytes was detected by the absorbance value.The results showed that the relative expression of MC1R mRNA in the siRNA2 and siRNA3 groups was significantly lower (P<0.01) than that in the blank control group，but there were no significant differences at the relative expression of MC1R mRNA in the siRNA1 and negative control group (P>0.05)；the production of melanin synthesis of siRNA2 and siRNA3 groups were significantly lower than that in the blank control group (P<0.01).The study was successfully transfected MC1R siRNA into alpaca melanocytes and the effective MC1R siRNA was screened from 3 groups of specific siRNAs.The inhibition of MC1R is consistent with the amount of melanin synthesis.MC1R of melanocytes played a regulatory role in the synthesis of melanin，and the mechanism need further research.

Key words: siRNA, MC1R gene, alpaca, melanocyte, melanin

中图分类号:

Q342

孙笑尉，张雪莲，庞全海，李振，赵礼军，陈娟娟，牛峰. RNAi对羊驼黑色素细胞中MC1R表达量及黑色素合成的影响[J]. 畜牧兽医学报, 2015, 46(7): 1163-1168.

SUN Xiao-wei，ZHANG Xue-lian，PANG Quan-hai，LI Zhen，ZHAO Li-jun，CHEN Juan-juan，NIU Feng. Effect of RNAi on MC1R Expression and Melanin Synthesis in the Melanocytes of Alpaca[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1163-1168.

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RNAi对羊驼黑色素细胞中MC1R表达量及黑色素合成的影响

Effect of RNAi on MC1R Expression and Melanin Synthesis in the Melanocytes of Alpaca

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