miR-186-5p对绵羊黑色素细胞迁移增殖的影响

doi:10.11843/j.issn.0366-6964.2017.11.007

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (11): 2068-2075.doi: 10.11843/j.issn.0366-6964.2017.11.007

miR-186-5p对绵羊黑色素细胞迁移增殖的影响

许冬梅^1,2, 赵宇军¹, 杜斌¹, 张亚玲¹, 朱芷葳², 谢建山³, 范瑞文¹, 于秀菊¹, 董常生^1*

1. 山西农业大学动物科技学院, 太谷 030801;
2. 山西农业大学生命科学学院, 太谷 030801;
3. 山西医科大学基础医学院, 太原 030001

收稿日期:2017-02-27 出版日期:2017-11-23 发布日期:2017-11-23
通讯作者: 董常生,教授,博士生导师,E-mail:cs_dong@sxau.edu.cn
作者简介:许冬梅(1974-),女,山西太谷人,博士生,主要从事分子生物学研究,E-mail:carrol-xv@sohu.com
基金资助:
山西农业大学团队创新项目（CXTN201201）；国家自然科学基金（31402156）；山西省攻关项目（2014031102-3）；山西省青年基金（2014021028-2）

The Influences of miR-186-5p on Migration and Proliferation of Sheep Melanocytes

XU Dong-mei^1,2, ZHAO Yu-jun¹, DU Bin¹, ZHANG Ya-ling¹, ZHU Zhi-wei², XIE Jian-shan³, FAN Rui-wen¹, YU Xiu-ju¹, DONG Chang-sheng^1*

1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
2. College of Life Science, Shanxi Agricultural University, Taigu 030801, China;
3. School of Basic Medical Sciences, Shanxi Medical University, Taiyuan 030001, China

Received:2017-02-27 Online:2017-11-23 Published:2017-11-23

摘要/Abstract

摘要：

旨在研究miR-186-5p对绵羊黑色素细胞迁移增殖的影响。本研究通过荧光定量PCR检测不同毛色绵羊皮肤中miR-186-5p的表达差异；运用细胞转染使miR-186-5p在绵羊黑色素细胞中过表达，然后通过荧光定量PCR检测miR-186-5p的表达，通过分光光度计测定转染细胞中黑色素含量，运用划痕试验判定黑色素细胞的增殖迁移能力。结果显示，miR-186-5p在绵羊不同毛色皮肤中均有表达，但白色皮肤表达高于黑色皮肤且差异显著（P<0.05）；在过表达miR-186-5p的绵羊黑色素细胞中，miR-186-5p的表达显著升高（P<0.01）；黑色素含量显著减少（P<0.05）；黑色素细胞的增殖和迁移能力有所降低。综上表明，miR-186-5p与绵羊皮肤黑色素的生成有关，绵羊黑色素细胞中过表达miR-186-5p降低了黑色素的生成，同时也抑制了绵羊黑色素细胞的迁移和增殖。

Abstract:

The aim of this study was to explore influence of miR-186-5p on migration and proliferation of sheep melanocytes. The expression of miR-186-5p in different color skin of sheep were determined by Quantitative real-time PCR; The over-expression vectors of miR-186-5p were transfected into sheep melanocytes, and then, the expression of miR-186-5p was analyzed using Quantitative real-time PCR; The melanin content were detected by spectrophotometer; The capacity of migration and proliferation were analyzed by wound-healing assay. The results showed that the expression of miR-186-5p was significantly higher in white skin than that in black skin (P<0.05); In transfected melanocytes with over-expression of miR-186-5p, the expression of miR-186-5p was significantly increased (P<0.01), the level of melanin content was significantly reduced(P<0.05),the capacity of migration and proliferation of melanocytes also reduced. In summary, miR-186-5p was associated with the formation of melanin in sheep skin, in sheep melanocytes, overexpression of miR-186-5p would reduce the melanin production and suppress the migration and proliferation of melanocytes.

中图分类号:

S826.2

许冬梅, 赵宇军, 杜斌, 张亚玲, 朱芷葳, 谢建山, 范瑞文, 于秀菊, 董常生. miR-186-5p对绵羊黑色素细胞迁移增殖的影响[J]. 畜牧兽医学报, 2017, 48(11): 2068-2075.

XU Dong-mei, ZHAO Yu-jun, DU Bin, ZHANG Ya-ling, ZHU Zhi-wei, XIE Jian-shan, FAN Rui-wen, YU Xiu-ju, DONG Chang-sheng. The Influences of miR-186-5p on Migration and Proliferation of Sheep Melanocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2068-2075.

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miR-186-5p对绵羊黑色素细胞迁移增殖的影响

The Influences of miR-186-5p on Migration and Proliferation of Sheep Melanocytes

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