山羊睾丸和附睾头β防御素家族的表达谱及生物信息学分析

doi:10.11843/j.issn.0366-6964.2015.07.007

摘要/Abstract

摘要：

为探明山羊睾丸和附睾头β防御素家族基因表达特点及其蛋白的特性。利用3只7日龄羔羊睾丸和3只成年种公羊睾丸和附睾头的Illumina HiSeq^TM 2000高通量转录组测序数据，筛选出β防御素家族基因并进行差异表达基因比较分析，利用生物信息学对β防御素家族蛋白特性进行预测。结果显示：在山羊7日龄睾丸、成年睾丸和附睾头中分别表达7、12和33种β防御素，其表达量最高的分别是gDB123、gDB119和gDB124，成年睾丸中特异性表达是gDB33，附睾头中特异表达有21种β防御素。山羊β防御素分子量在6.89~13.85 ku，为小分子、极性、带正电荷、疏水性的强碱性多肽。山羊β防御素也是含高度保守6个半胱氨酸的空间构象，基本结构-C-X_2-45-C-X_3-6-C-X_3-13-C-X_4-7-CC-，其中24种β防御素结构为-C-X_5，6-C-X_3，4-C-X₉-C-X_5，6-CC-。有16种β防御素是分泌型糖蛋白，除gDB126无磷酸化位点外，其余的β防御素蛋白均有磷酸化位点。山羊附睾头特异性高度表达有丰富β防御素，它们是一类抗微生物的阳离子肽，在精子成熟过程中形成精子膜上糖被，或精子运动获得的过程中发挥重要作用；也可作为信号分子，某些信号通路中发挥作用。

关键词: &beta, 防御素家族, 表达谱, 生物信息, 睾丸, 附睾, 公山羊

Abstract:

The study was conducted to investigate the expression characteristics and bioinformatics analysis of beta-defensin family genes in the testes and epididymal caput of bucks.The data were from the transcriptomes of three testes of 7-day-old kids，testes and epididymal caput of three adult bucks of known fertility analyzed by Illumina HiSeq^TM2000 high-throughput RNA sequencing.All beta-defensin family genes were sorted out according to annotation of unigenes and contigs for analysis of different expression.Protein profile of beta-defensins was analyzed by bioinformatics methods.The results showed that a total of 7，12 and 33 β-defensins were discovered in testes of 7-day-old kids，testes and epididymal caput of adult bucks respectively，while gDB123，gDB119 and gDB124 were the highest expression in the testes of 7-day-old kids，testes and epididymal caput of adult bucks respectively.gDB33 was specifically expressed in testes of adult bucks.Twenty-one β-defensins were specifically expressed in epididymal caput of adult bucks.The molecular weight of β-defensins was from 6.89 to 13.85 ku.The bioinformatics analysis showed that goat β-defensins protein were small molecular，polar，hydrophobicity and basic polypeptide with positive charge.A high degrees of conservation in spacing pattern was 6 cysteines in peptides sequence of β-defensins，which consensus pattern was -C-X_2-45-C-X_3-6-C-X_3-13-C-X_4-7-CC-.However，the pattern of 24 β-defensins was -C-X_5，6-C-X_3，4-C-X₉-C-X_5，6-CC-.A total of 16 β-defensins were secreted glycoprotein.Except for gDB126，the rest of 24 β-defensins had the phosphorylation sites.The goat β-defensins family protein were also the antimicrobial cationic peptides.The rich specific-epididymis β-defensins were expressed in epididymal caput，which could coat sperm plasma membrane with glycocalyx in sperm maturation and play a vital role in making sperm motion.And β-defensins maybe were cell signal in some pathways.

Key words: β-defensins family, expression profiles, bioinformatics, testis, epididymal caput, bucks

中图分类号:

S827.2

张春香，杜海燕，张彩霞，郑亚琳，任有蛇，姜玉锁. 山羊睾丸和附睾头β防御素家族的表达谱及生物信息学分析[J]. 畜牧兽医学报, 2015, 46(7): 1122-1133.

ZHANG Chun-xiang，DU Hai-yan，ZHANG Cai-xia，ZHENG Ya-lin，REN You-she，JIANG Yu-suo. Expression Profiles and Bioinformatics Analysis of beta-defensin Family Genes in the Testes and Epididymal Caput of Bucks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1122-1133.

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