绵羊UCP2基因的克隆及其在脂肪组织中的季节性差异表达

doi:10.11843/j.issn.0366-6964.2015.07.006

摘要/Abstract

摘要：

旨在研究绵羊UCP2基因的克隆和在脂肪组织中的季节性表达差异，以揭示季节对UCP2基因表达的调控作用。本研究采用RT-PCR和RACE法克隆UCP2 cDNA全长序列。以南非肉用美利奴和山西肉用绵羊为对象，用real-time PCR和免疫组化技术检测UCP2 mRNA及其编码蛋白在冬春和夏秋2个阶段6种脂肪组织（皮下脂肪、肠系膜、大网膜、小网膜、腹膜后脂肪和肾周脂肪）中的差异表达。结果表明，绵羊UCP2 cDNA全长1 641 bp，包括8个外显子，开放阅读框930 bp，编码309个氨基酸。UCP2 mRNA在6种脂肪组织中均有表达，其中肾周脂肪中的表达量最高，皮下脂肪中最低，即深层脂肪中的表达量高于浅层脂肪（P<0.05）。UCP2蛋白的组织表达趋势与mRNA相似，且均分布于细胞膜上。二者的表达均受季节的显著影响，冬春阶段的表达量高于夏秋季节（P<0.05）。这些结果说明绵羊脂肪组织中UCP2基因的表达与季节有关，以维持体温和调节机体能量平衡。

关键词: 绵羊, UCP2基因, 基因表达, 脂肪组织, 季节性

Abstract:

This study focused on the cloning of ovine UCP2 gene and its seasonal expression differences in adipose tissues.The results would reveal the effects of seasonal regulation on the UCP2 expression.The full-length cDNA was cloned by RACE method.Real-time PCR and immunohistochemistry techniques were used to study the expressions of UCP2 mRNA and its coding protein in 6 adipose tissues of South African Mutton Merino and Shanxi Mutton Sheep Dam Line during two season periods.The results showed that the ovine UCP2 cDNA was 1 641 bp in length with a 930 bp open reading frame.It contained 8 exons which encoded 309 amino acids.The mRNA expressed in all 6 adipose tissues detected in this study.Higher expression in deep than in superficial depots (P<0.05) was found as suggested by the highest expression in perirenal and lowest in subcutaneous fat.UCP2 expressed in similar trend at mRNA and protein levels，and distributed on the membrane of adipocytes.Both mRNA and protein expressions were higher in winter-spring period than that in summer-autumn period (P<0.05).These results indicate that UCP2 differentially expression in adipose tissues is related to seasonal changes and functions in maintaining body temperature and regulating energy balance.

Key words: sheep, UCP2 gene, gene expression, adipose tissues, seasonality

中图分类号:

S826.2

刘建华，乔利英，郭云雁，贾夏丽，刘文忠. 绵羊UCP2基因的克隆及其在脂肪组织中的季节性差异表达[J]. 畜牧兽医学报, 2015, 46(7): 1114-1121.

LIU Jian-hua，QIAO Li-ying，GUO Yun-yan，JIA Xia-li，LIU Wen-zhong. Cloning of Ovine UCP2 Gene and Its Seasonally Differential Expression in Adipose Tissues[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1114-1121.

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