雏鸭感染鹅源H5N1亚型禽流感病毒后免疫器官T细胞免疫功能及IL-2 mRNA转录变化

doi:10.11843/j.issn.0366-6964.2014.09.016

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (9): 1497-1504.doi: 10.11843/j.issn.0366-6964.2014.09.016

雏鸭感染鹅源H5N1亚型禽流感病毒后免疫器官T细胞免疫功能及IL-2 mRNA转录变化

于学武^1,2，吕晓萍¹，刘超男¹，高雪丽¹，石霖^1,2，刘明³，郑世民^1*

（1.东北农业大学动物医学学院，哈尔滨 150030；2.辽宁省动物疫病预防控制中心，沈阳 110164；3.中国农业科学院哈尔滨兽医研究所，哈尔滨 150001）

收稿日期:2014-01-25 出版日期:2014-09-23 发布日期:2014-09-23
通讯作者: 郑世民，E-mail：zhengshiminbl@sohu.com
作者简介:于学武（1982-），男，黑龙江尚志人，博士，主要从事动物疫病防控和免疫病理研究，E-mail：yuxuewu1@163.com
基金资助:
国家自然科学基金（30371066）；黑龙江省自然科学基金（C200841）

Changes of T Lymphocytes Proliferation Function，Quantity of T_ANAE⁺ Cells and Transcription of IL-2 mRNA in Immune Organs of Ducks Infected with H5N1 Subtype Avian Influenza Virus Isolated from Geese

YU Xue-wu^1,2，LYU Xiao-ping¹，LIU Chao-nan¹，GAO Xue-li¹，SHI Lin^1,2，LIU Ming³，ZHENG Shi-min^1*

（1.College of Veterinary Medicine，Northeast Agricultural University，Harbin 150030， China;2.Animal Epidemic Diseases Control and Prevention Center of Liaoning Province，Shenyang 110164，China；3.Harbin Veterinary Research Institute，Chinese Academy of Agricultural Sciences，Harbin 150001，China）

Received:2014-01-25 Online:2014-09-23 Published:2014-09-23

摘要/Abstract

摘要：

为探讨T细胞及白细胞介素（IL）-2在禽流感免疫、发病机制中的作用，利用细胞培养技术、四甲基偶氮唑盐（MTT）检测法、组织石蜡切片及α-醋酸萘酯酶染色法和实时荧光PCR技术，对雏鸭感染H5N1亚型禽流感病毒（AIV）后，其免疫器官T淋巴细胞增殖功能及数量和IL-2 mRNA转录的动态变化进行全面系统的研究。结果发现，H5N1亚型AIV感染21日龄雏鸭后，其免疫器官IL-2 mRNA转录、T淋巴细胞数量及其对刀豆蛋白A（ConA）的增殖功能在感染的急性期均显著低于对照雏鸭，表明AIV感染雏鸭免疫器官细胞因子的免疫调节功能降低，免疫活性细胞，特别是T淋巴细胞活化、增殖受阻，是导致机体细胞免疫功能低下的主要原因，也可能是雏鸭在感染早期发生死亡的重要因素。

关键词: 雏鸭, H5N1亚型禽流感病毒, 免疫器官, 细胞免疫, IL-2

Abstract:

Technology of cell culture，cyto-histochemistry staining and real-time fluorescence quantitative PCR （SYBR GreenⅠ） were used to research dynamic changes of the proliferation responses of T-lymphocytes to ConA，the quantity of T_ANAE⁺ cells and the transcription of IL-2 mRNA in immune organs of ducks infected with AIV at 21 days old，which were useful to reveal the mechanism and regulation of immunity.The experiment provided scientific evidence for the mechanism of morbidity and useful effective prevention.The results were as follows：the transcription of IL-2 mRNA，the proliferation responses of T-lymphocytes to ConA and the quantity of T_ANAE⁺ cells in immune organs were all decreased remarkably in initial period of AIV infection.And it indicated that molecular immune function of cytokines was inhibited，and the activation and proliferation of the immunocompetent cells，especially T-lymphocyte，were inhibited as well.The cellular immunity functions were much lower than that of controls，which could be the main reasons of the ducks death in initial period of AIV infection.

Key words: duck, H5NI AIV, immune organ, cellular immune, IL-2

中图分类号:

S852.659.5

于学武，吕晓萍，刘超男，高雪丽，石霖，刘明，郑世民. 雏鸭感染鹅源H5N1亚型禽流感病毒后免疫器官T细胞免疫功能及IL-2 mRNA转录变化[J]. 畜牧兽医学报, 2014, 45(9): 1497-1504.

YU Xue-wu，LYU Xiao-ping，LIU Chao-nan，GAO Xue-li，SHI Lin，LIU Ming，ZHENG Shi-min. Changes of T Lymphocytes Proliferation Function，Quantity of T_ANAE⁺ Cells and Transcription of IL-2 mRNA in Immune Organs of Ducks Infected with H5N1 Subtype Avian Influenza Virus Isolated from Geese[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(9): 1497-1504.

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雏鸭感染鹅源H5N1亚型禽流感病毒后免疫器官T细胞免疫功能及IL-2 mRNA转录变化

Changes of T Lymphocytes Proliferation Function，Quantity of T_ANAE⁺ Cells and Transcription of IL-2 mRNA in Immune Organs of Ducks Infected with H5N1 Subtype Avian Influenza Virus Isolated from Geese

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雏鸭感染鹅源H5N1亚型禽流感病毒后免疫器官T细胞免疫功能及IL-2 mRNA转录变化

Changes of T Lymphocytes Proliferation Function，Quantity of TANAE+ Cells and Transcription of IL-2 mRNA in Immune Organs of Ducks Infected with H5N1 Subtype Avian Influenza Virus Isolated from Geese

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Changes of T Lymphocytes Proliferation Function，Quantity of T_ANAE⁺ Cells and Transcription of IL-2 mRNA in Immune Organs of Ducks Infected with H5N1 Subtype Avian Influenza Virus Isolated from Geese