杜寒杂交繁殖母羊氮代谢和维持净蛋白质需要的研究

doi:10.11843/j.issn.0366-6964.2014.06.012

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (6): 943-952.doi: 10.11843/j.issn.0366-6964.2014.06.012

杜寒杂交繁殖母羊氮代谢和维持净蛋白质需要的研究

楼灿，姜成钢，马涛，纪守坤，陈丹丹，屠焰，张乃锋，刁其玉^*

（中国农业科学院饲料研究所，农业部饲料生物技术重点开放试验室，北京 100081）

收稿日期:2013-10-08 出版日期:2014-06-23 发布日期:2014-06-23
通讯作者: 刁其玉，研究员，博士生导师，E-mail：diaoqiyu@caas.cn
作者简介:楼灿（1988-），男，浙江浦江人，硕士，主要从事反刍动物营养研究，E-mail：loucan816@sina.com
基金资助:
国家现代肉羊产业技术体系（CARS-39）

Study of Nitrogen Metabolism and Net Protein Requirement for Maintenance of Dorper×Thin-tailed Han Crossbred Breeding Ewes

LOU Can，JIANG Cheng-gang，MA Tao，JI Shou-kun，CHEN Dan-dan，TU Yan，ZHANG Nai-feng，DIAO Qi-yu^*

（Key Laboratory of Feed Biotechnology of Ministry of Agriculture,Feed Research Institute,Chinese Academy of Agricultural Sciences，Beijing 100081, China）

Received:2013-10-08 Online:2014-06-23 Published:2014-06-23

摘要/Abstract

摘要：

本试验用日粮饲喂量梯度方法研究杜寒杂交母羊不同生理时期氮的表观消化代谢规律和维持的净蛋白质需要。15只同期发情和人工授精妊娠母羊按体重分为3组：1）自由采食组，2）80%自由采食组，3）60%自由采食组；另有9只空怀母羊做对照。消化代谢试验分别在空怀期，妊娠期 40、100和130 d，哺乳期20、50和80 d进行，得到母羊不同时期的氮表观代谢和维持净蛋白质需要参数。氮的表观消化率在空怀期,妊娠40、100、130 d及哺乳20、50、80 d时分别为45.21%～51.34%；59.71%～64.14%、69.98%～71.91%、67.34%～68.80%；73.72%～82.71%、72.79%～80.51%、73.57%～76.47%。在空怀期和哺乳中、后期存在显著差异（P＜0.01）；空怀期、妊娠40、100、130 d及哺乳20、50、80 d时母羊氮采食量（N intake，g•kg^-1 W^0.75•d^-1）和氮沉积量（RN，g•kg^-1 W^0.75•d^-1）之间的回归方程分别为RN=0.341 5NI-0.269 7；RN=0.489 5NI-0.303 7、RN=0.384 5 NI-0.323 4、RN=0.457 4NI-0.496 2；RN=0.252 1NI-0.338 0、RN=0.282 1NI-0.313 6、RN=0.286 3NI-0.298 2（P＜0.01）。母羊空怀期、妊娠40、100、130 d及哺乳20、50、80 d 7个阶段的维持净氮需要分别为269.7、303.7、323.4、496.2、338.0、313.6和298.2 mg•kg^-1 W^0.75•d^-1。

关键词: 杂交繁殖母羊, 妊娠期, 哺乳期, 氮消化代谢, 维持净蛋白质需要

Abstract:

This experiment was conducted to investigate the nitrogen apparent digestibility of different feeding levels and net protein requirement for maintenance of Dorper × Thin-tailed Han crossbred ewes in different physiological periods.According to the weight,fifteen ewes with synchronous estrus and artificial insemination were assigned into three groups，one group fed ad libitum(100%),and the other two groups fed eighty percents(80%) or sixty percents(60%) of ad libitum,and another nine non-pregnant ewes were assigned into blank control group.Digestibility trials was performed in the non-pregnant condition and 40th,100th,130th day of pregnancy and 20th,50th,80th day of lactation,to determine the metabolic parameters of nitrogen and net protein requirement for maintenance.The apparent digestibilities of nitrogen in the non-pregnancy and 40th,100th,130th day of pregnancy and 20th,50th,80th day of lactation were 45.21%-51.34%；59.71%-64.14%,69.98%-71.91%,67.34%-68.80%；73.72%-82.71%,72.79%-80.51%,73.57%-76.47%，respectively，which only in the non-pregnancy,mid- and late-lactation had significant differences (P＜0.01).The regression equations between nitrogen intake (N intake，g•kg^-1 W^0.75•d^-1) and nitrogen retention (RN，g•kg^-1 W^0.75•d^-1) in the non-pregnancy and 40th,100th,130th day of pregnancy and 20th,50th,80th day of lactation were RN=0.341 5NI-0.269 7；RN=0.489 5NI-0.303 7,RN=0.384 5NI-0.323 4,RN=0.457 4NI-0.496 2；RN=0.252 1NI-0.338 0,RN=0.282 1NI-0.313 6,RN=0.286 3NI-0.298 2(P＜0.01)，respectively.The net nitrogen requirements for maintenance of ewes in the non-pregnancy and 40th,100th,130th day of pregnancy and 20th,50th,80th day of lactation were 269.7,303.7,323.4,496.2,338.0,313.6 and 298.2 mg•kg^-1 W^0.75•d^-1 ，respectively.

Key words: crossbred breeding ewes, pregnancy, lactation, digestion and metabolism of nitrogen, net protein requirement for maintenance

中图分类号:

S826
S816.32

楼灿，姜成钢，马涛，纪守坤，陈丹丹，屠焰，张乃锋，刁其玉. 杜寒杂交繁殖母羊氮代谢和维持净蛋白质需要的研究[J]. 畜牧兽医学报, 2014, 45(6): 943-952.

LOU Can，JIANG Cheng-gang，MA Tao，JI Shou-kun，CHEN Dan-dan，TU Yan，ZHANG Nai-feng，DIAO Qi-yu. Study of Nitrogen Metabolism and Net Protein Requirement for Maintenance of Dorper×Thin-tailed Han Crossbred Breeding Ewes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 943-952.

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杜寒杂交繁殖母羊氮代谢和维持净蛋白质需要的研究

Study of Nitrogen Metabolism and Net Protein Requirement for Maintenance of Dorper×Thin-tailed Han Crossbred Breeding Ewes

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