不同蛋白质饲料对宣汉黄牛瘤胃固相粘附蛋白分解菌数量的影响

doi:10.11843/j.issn.0366-6964.2014.06.013

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (6): 953-959.doi: 10.11843/j.issn.0366-6964.2014.06.013

不同蛋白质饲料对宣汉黄牛瘤胃固相粘附蛋白分解菌数量的影响

吴小燕，王之盛^*，邹华围

（四川农业大学动物营养研究所，教育部动物抗病营养重点实验室，雅安 625014）

收稿日期:2013-11-14 出版日期:2014-06-23 发布日期:2014-06-23
通讯作者: 王之盛，教授，博士生导师，主要从事反刍动物营养与饲料研究，E-mail：wangzs67@163.com
作者简介:吴小燕（1987-），女，四川绵阳人，硕士生，主要从事反刍动物营养与饲料研究，E-mail：812181840@qq.com
基金资助:
国家肉牛牦牛产业技术体系(CARS-38)；农业部公益性行业专项饲料营养价值与畜禽饲养标准研究与应用(20090300608)

Different Protein Sources Dietary Affect the Quantities of Rumen Proteobacteria Adhensive to Solid Fractions in Xuanhan Yellow Cattle

WU Xiao-yan，WANG Zhi-sheng^*，ZOU Hua-wei

(Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education，Institute of Animal Nutrition，Sichuan Agricultural University，Ya’an 625014， China）

Received:2013-11-14 Online:2014-06-23 Published:2014-06-23

摘要/Abstract

摘要：

旨在研究豆粕、菜粕和棉粕3种蛋白质饲料对四川宣汉黄牛瘤胃固相粘附蛋白分解菌［嗜淀粉瘤胃杆菌(R.amylophilus)、溶纤维丁酸弧菌(B.fibrisolvens)、瘤胃普雷沃氏菌(P.ruminicoal)和牛链球菌(S.bovis)］数量的影响。选用4头健康且体重相近装有永久瘤胃瘘管的宣汉黄牛，采用4×4的拉丁方设计，共4期，每期10 d，分别饲喂以豆粕（SBM）、菜粕（RSM）和棉粕（CSM）替换基础日粮（CN）精料15%的日粮，采用PCR技术分析蛋白分解菌数量的变化。结果表明：1）豆粕组嗜淀粉瘤胃杆菌、溶纤维丁酸弧菌、栖瘤胃普雷沃氏菌和牛链球菌的数量分别在采食后9、9、5和5 h时达到最大值，菜粕组分别在采食后3、7、7和1 h时达到最大值，棉粕组分别在采食后9、9、7和1 h时达到最大值；2）豆粕组嗜淀粉瘤胃杆菌数量的平均值最高，棉粕组最低（P<0.05）；棉粕组溶纤维丁酸弧菌、栖瘤胃普雷沃氏菌和牛链球菌数量的平均值最高，豆粕组最低（P<0.05）。综上，不同蛋白质饲料显著影响瘤胃固相粘附蛋白分解菌的数量，以棉粕对瘤胃固相粘附蛋白分解菌的促进效应最好。

关键词: 菜粕, 豆粕, 棉粕, 宣汉黄牛, 蛋白分解菌

Abstract:

The objective of this paper was to investigate the effects of three protein feeds (soybean meal，rapeseed meal and cottonseed meal) on quantities of ruminal proteobacteria (R.amylophilus，B.fibrisolvens,P.ruminicoal and S.bovis) adhered to solid fractions in Sichuan Xuanhan Yellow cattle.Four rumen-cannulated Xuanhan Yellow cattle with similar healthy and weight were each fed four diets with different protein feeds over a period in a 4×4 Latin Square design，which were basal diet(CN)，replaced 15% concentration of basal diet with soybean meal (SBM)，rapeseed meal (RSM) and cottonseed meal (CSM)，respectively.The PCR technique was used to analyze the change of the population of ruminal proteobacteria adhensive to solid fractions.The results showed that: 1)the quantities of R.amylophilus，B.fibrisolvens，P.ruminicoal and S.Bovis in group SBM reached the highest point at 9，9，5 and 5 h after morning feeding，the group RSM at 3，7，7 and 1 h，the group CSM at 9，9，7 and 1 h.2)the highest mean value was found in group SBM while the lowest in the group CSM on R.amylophilus(P<0.05) quantity; the highest mean value was recorded in group CSM while the lowest in SBM on the quantities of B.fibrisolvens，P.ruminicoal and S.Bovis (P<0.05) quantities.In conclusion，different protein feeds significantly affected the quantities of ruminal proteobacteria，and cottonseed meal had a better promoting effect on ruminal proteobacteria adhensive to solid fractions.

Key words: rapeseed meal, soybean meal, cottonseed meal, Xuanhan Yellow cattle, ruminal proteobacteria

中图分类号:

S823
S816.4

吴小燕，王之盛，邹华围. 不同蛋白质饲料对宣汉黄牛瘤胃固相粘附蛋白分解菌数量的影响[J]. 畜牧兽医学报, 2014, 45(6): 953-959.

WU Xiao-yan，WANG Zhi-sheng，ZOU Hua-wei. Different Protein Sources Dietary Affect the Quantities of Rumen Proteobacteria Adhensive to Solid Fractions in Xuanhan Yellow Cattle[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 953-959.

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不同蛋白质饲料对宣汉黄牛瘤胃固相粘附蛋白分解菌数量的影响

Different Protein Sources Dietary Affect the Quantities of Rumen Proteobacteria Adhensive to Solid Fractions in Xuanhan Yellow Cattle

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