围产期山羊血钙稳恒与胃肠道CaBP-D9k表达对不同日粮阴阳离子差的响应研究

doi:10.11843/j.issn.0366-6964.2014.06.011

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (6): 932-942.doi: 10.11843/j.issn.0366-6964.2014.06.011

围产期山羊血钙稳恒与胃肠道CaBP-D9k表达对不同日粮阴阳离子差的响应研究

杨艺¹，张吉鹍²，莘海亮¹，田兴舟¹，韩晓洁¹，段永邦¹，王代林³，吴文旋^1*，李胜利^4*

（1.贵州大学动物科学学院，贵阳 550025； 2.江西省农业科学院畜牧兽医研究所，南昌 330200；3.贵州省长顺县敦操乡，长顺 550700；4.动物营养学国家重点实验室，北京 100193）

收稿日期:2013-11-27 出版日期:2014-06-23 发布日期:2014-06-23
通讯作者: 吴文旋，教授，E-mail：wwx3419@126.com；李胜利，教授，博导，E-mail：lisheng0677@163.com
作者简介:杨艺（1989-），贵州贵阳人，硕士，主要从事反刍动物营养研究，E-mail：yangyi19890209@gmail.com
基金资助:
国家自然科学基金（30901038；31160468；31360658）；动物营养学国家重点实验室开放课题（2004DA125184F1115）

Responses of Blood Calcium and Calcium Binding Proteins-D9k Expression Level to Dietary Cation-anion Difference of Transition Goats

YANG Yi¹，ZHANG Ji-kun²，XIN Hai-liang¹，TIAN Xing-zhou¹，HAN Xiao-jie¹，DUAN Yong-bang¹，WANG Dai-lin³，WU Wen-xuan^1*，LI Sheng-li^4*

(1.College of Animal Science，Guizhou University，Guiyang 550025， China； 2.Institute of Animal Science, Jiangxi Academy of Agricultural Science, Nanchang 330200， China； 3.Duncao Township，Changshun County，Changshun 550700， China;4.Key Laboratory of Animal Nutrition,Beijing 100193,China)

Received:2013-11-27 Online:2014-06-23 Published:2014-06-23

摘要/Abstract

摘要：

本试验旨在研究不同日粮阴阳离子差（Dietary cation-anion difference，DCAD）对围产期山羊血浆Ca²⁺和胃肠道钙结合蛋白9kDa（Calcium binding proteins 9kDa，CaBP-D9k）表达水平的影响，探索低DCAD日粮预防围产期奶牛低血钙的可能机制。将27头围产期贵州黑山羊随机分为3组，每组9头，分别饲喂3组DCAD日粮：高DCAD（HD，+325）日粮组、对照组（CON，+179）、低DCAD（LD，-110）日粮组。在产前10 d、产羔当天、产后3 d分别采血并屠宰山羊，检测其血浆离子指标与胃肠道CaBP-D9k表达水平。结果表明，与高DCAD日粮相比，低DCAD日粮不影响山羊采食，酸化了山羊尿液pH（P<0.05），提高了整个试验期内血浆Cl^-水平（P<0.05）。孕期内，山羊血浆Ca²⁺浓度随日粮DCAD水平的降低而增加（P<0.05），采食低DCAD日粮导致了更高的产后血浆Ca²⁺浓度（P<0.05）。除回肠、盲肠及直肠外，低DCAD日粮较高DCAD日粮明显上调山羊胃肠道其余区段内CaBP-D9k mRNA表达水平（P<0.05），并缓和了产后皱胃、空肠与回肠CaBP-D9k mRNA表达水平的降幅（P<0.05）。由此可见，低DCAD日粮有利于维持围产期山羊血浆Ca²⁺稳恒，伴随胃、十二指肠、空肠及结肠肠段CaBP-D9k mRNA表达水平的上调，表明上述区段的钙吸收能力得到增强。这可能是低DCAD日粮有效维持围产期奶牛血浆Ca²⁺稳恒的作用机制。

关键词: 日粮阴阳离子差, 血钙, 钙结合蛋白9kDa, 围产期山羊

Abstract:

The study was conducted to clarify the mechanism of low dietary cation-anion difference (DCAD) level preventing hypocalcemia in transition cows by investigating the influence of DCAD levels on blood Ca concentration and calcium binding protein-9kDa (CaBP-D9k) expression level in gastrointestinal tract of transition goats.Twenty-seven Guizhou Black goats in transition period were randomly allocated to 3 treatments with 9 goats each treatment and were fed 3 diets with varying DCAD levels：high DCAD (HD，+325)，control (CON，+179) and low DCAD (LD，-110) groups，respectively.Goats were blooded on d 10 before lambing，d 0 for lambing and d 3 after lambing，respectively，and were slaughtered to detect the plasma parameters and CaBP-D9k expression level of gastrointestinal tract.Compared with high DCAD，feed intake was unaffected by low DCAD treatment (P>0.05)，urine pH of goats fed LD was reduced with decreasing DCAD level (P<0.05).Compared to CON and HD,reducing DCAD level induced higher plasma Cl^- and Ca²⁺ concentration of goats during the experiment (P<0.05).Greater CaBP-D9k mRNA expression level was observed in whole gastrointestinal tract of LD-fed goats except for ileum，cecum and rectum compared with goats in HD group (P<0.05).Moreover，feeding LD diet relieved the decline of CaBP-D9k mRNA expression level in abomasum，jejunum and colon after lambing (P<0.05).In conclusion，reducing DCAD could up-regulate CaBP-D9k expression level in stomach，proximal intestine and colon，and kept Ca²⁺ concentration for transition goats.The results indicated that Ca²⁺ absorption in the above gastrointestinal tracts was increased and would be the possible mechanism of low DCAD enhancing blood Ca homeostasis in dairy cows during periparturient period.

Key words: dietary cation-anion difference, blood calcium, calcium binding protein-9kDa, transition goats

中图分类号:

S827
S816.32

杨艺，张吉鹍，莘海亮，田兴舟，韩晓洁，段永邦，王代林，吴文旋，李胜利. 围产期山羊血钙稳恒与胃肠道CaBP-D9k表达对不同日粮阴阳离子差的响应研究[J]. 畜牧兽医学报, 2014, 45(6): 932-942.

YANG Yi，ZHANG Ji-kun，XIN Hai-liang，TIAN Xing-zhou，HAN Xiao-jie，DUAN Yong-bang，WANG Dai-lin，WU Wen-xuan，LI Sheng-li. Responses of Blood Calcium and Calcium Binding Proteins-D9k Expression Level to Dietary Cation-anion Difference of Transition Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 932-942.

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围产期山羊血钙稳恒与胃肠道CaBP-D9k表达对不同日粮阴阳离子差的响应研究

Responses of Blood Calcium and Calcium Binding Proteins-D9k Expression Level to Dietary Cation-anion Difference of Transition Goats

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