GnRH受体在山羊腹腔肠系膜前神经节的分布及其意义

摘要/Abstract

摘要：

本研究旨在观察促性腺激素释放激素受体(Gonadotropin-releasing hormone receptor，GnRHR)在山羊腹腔肠系膜前神经节的分布特点，以探讨GnRH是否可以影响腹腔肠系膜前神经节(Celiac-superior mesenteric ganglia，CSMG)的活动。取雄性和雌性成年山羊的腹腔肠系膜前神经节各5个，经免疫组织化学SP法染色后，观察GnRHR在肠系膜前神经节的分布特点，并用Image-Pro Plus 6.0 (IPP 6.0)软件图像半定量分析技术，分析腹腔肠系膜前神经节中的神经元和非神经元的GnRHR分布差异。结果表明：GnRHR强阳性产物主要分布神经元的胞膜和胞质中；血管内皮细胞、神经纤维和卫星细胞中只有GnRHR弱阳性产物；GnRHR在神经细胞和非神经细胞中的表达量呈极显著性差异(P<0.01)。上述结果表明，腹腔肠系膜前神经节对GnRH具有反应性，且神经元为其作用的靶细胞，提示GnRH可能通过影响腹腔肠系膜前神经节神经元的活动，进而影响其发出的交感节后神经纤维所支配的靶器官功能这一途径来调节胃肠的生理活动。

关键词: GnRH受体, 腹腔肠系膜前神经节, 免疫组织化学SP法, 山羊

Abstract:

This experiment was conducted to detect existence of Gonadotropin-releasing hormone receptor (GnRHR) in the celiac-superior mesenteric ganglia (CSMG) of goats, and determine the possible influence of GnRH on CSMG. Five CSMG were taken from mature female and male goats， respectively. The distribution characteristics of GnRHR were observed after immunohistochemical SP staining. Image-Pro Plus 6.0 (IPP 6.0) was used as the method of semi-quantitative image analysis to evaluate expression difference of GnRHR between neurons and non-neuron cells. The results showed that strong GnRHR immunoreactivity (GnRHR-ir) products mainly distributed on the membrane and whole cytoplasm of neurons and weak GnRHR-ir products on non-neuron cells. Expression difference of GnRHR between these two sorts of cells was highly significant (P<0.01). The wide distribution of GnRHR in CSMG suggests that GnRH can exert potential effects on CSMG and neurons are the major targets, which implies GnRH may regulate the functional activities of the gastrointestinal system by sympathetic nerve from celiac superior mesenteric ganglion.

Key words: GnRHR, celiac-superior mesenteric ganglia, immunohistochemical SP method, goat

中图分类号:

S852.1

范洁，陈文东，郭晓，王志豪，明佳，郭瑜龙，徐永平. GnRH受体在山羊腹腔肠系膜前神经节的分布及其意义[J]. 畜牧兽医学报.

FAN Jie, CHEN Wen-dong, GUO Xiao, WANG Zhi-hao, MING Jia, GUO Yu-long, XU Yong-ping. Distribution of GnRH Receptor in Celiac-superior Mesenteric Ganglia of Goat and Its Implications[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA.

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