关键词: 猪圆环病毒2型, 油佐剂, 氢氧化铝佐剂, 灭活苗

Abstract: Porcine circovirus 2 was inactivated with 0.2% formaldehyde under 37℃, then was passaged on PK-15 cell three times and the inactivation effect was estimated by PCR method. The immune efficacies of the oil-adjuvant and the aluminum hydroxide-adjuvant inactivated vaccines against PCV2 were evaluated in mice. Mice were vaccinated subcutaneously twice at a threeweek interval. The specific ELISA antibody against PCV2 were detected at the 21st day after the first immunization and at the 14th day after the boost. In order to compare the immune efficacy between the homemade oilbased and imported oil-based vaccines, piglets were immunized with two inactivated PCV2 vaccines and the immune efficay was evaluated by presence or absence of seroconversion, clinical signs (fever), gross lesions, growth parameters and viremia. The results were as follows: ① PCV2 was inactivated completely with 0.2% formaldehyde after incubation for 16 h. ② The good immune response could be induced by two vaccines in mice and the immune efficacy of the oil-adjuvant inactivated vaccine was higher than that of the aluminum hydroxide-adjuvant inactivated vaccine. ③ High ELISA antibody titers of two oil-adjuvant vaccines could be detected in piglets after the boost. After challenge the rectal temperatures of all piglets were recorded. Average temperature of piglets in challenge control group was higher than that of immunized piglets and no pyrexia was observed in mock piglets. The average weight was increased in two immunized groups and mock group, but decreased in challenge control group. Comparison of average daily weight growth (ADWG) of vaccinated pigs with that of mock pigs revealed statistically significant difference between them (P<0.05). The fact that two vaccines could reduce the persistence time of PCV2 in piglets was indicated by detection of viremia and PCV2 in different tissues of challenged piglets. It could be concluded that inactivated PCV2 vaccines could induce immune response in mice and piglets and could protect piglets from PCV2 infection.

Key words: porcine circovirus 2, oil adjuvant, aluminum hydroxide adjuvant, inactivated vaccine